PMID- 18246712
OWN - NLM
STAT- MEDLINE
DCOM- 20080226
LR  - 20121115
IS  - 1522-6514 (Print)
IS  - 1522-6514 (Linking)
VI  - 9
IP  - 1
DP  - 2007 Jan-Feb
TI  - Analysis of gene expression in poplar trees (Populus deltoides x nigra, DN34) 
      exposed to the toxic explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX).
PG  - 15-30
LID - 10.1080/15226510601139375 [doi]
AB  - Poplar plants (Populus deltoides x nigra, DN34) growing under hydroponic 
      conditions were exposed to 50 mg L(-1) of hexahydro-1,3,5-trinitro-1,3,5-triazine 
      (RDX) for 24 h. The expression of genes potentially involved in the metabolism of 
      toxic explosives was analyzed by reverse-transcriptase (RT) real-time PCR. Genes 
      under study were selected by reference to corresponding genes that were 
      previously shown to be upregulated in the model plant Arabidopsis thaliana by 
      exposure to 2,4,6-trinitrotoluene (TNT) (Ekman et al., 2003. Plant Physiol., 133, 
      1397-1406). The target genes investigated include several genes encoding for 
      enzymes known to be involved in the detoxification of xenobiotic pollutants, such 
      as glutathione S-transferases (GSTs), cytochrome P-450s (CYPs), NADPH-dependent 
      reductases, and peroxidases. Starting from A. thaliana TNT-inducible genes, 
      corresponding Populus sequences were retrieved from the JGI Poplar Genome Project 
      database and were used to design gene-specific primers. 18S ribosomal DNA (rDNA) 
      was used as an internal standard and recorded gene expression levels were 
      normalized by reference to nonexposed plants. In three separate experiments, five 
      genes were found to be significantly amplified in leaf tissues by exposure to 
      RDX, including GST (9.7 fold), CYP (1.6 fold), reductases (1.6-1.7 fold), and 
      peroxidase (1.7 fold). In root tissues, only a single GST gene was found to be 
      significantly amplified by exposure to RDX (2.0 fold). These results show, for 
      the first time, that the exposure of poplar plants to RDX results in the 
      induction of several genes that are potentially involved in explosive 
      detoxification.
FAU - Tanaka, Sachiyo
AU  - Tanaka S
AD  - Department of Civil and Environmental Engineering, The University of Iowa, Iowa 
      City, Iowa, USA.
FAU - Brentner, Laura B
AU  - Brentner LB
FAU - Merchie, Kate M
AU  - Merchie KM
FAU - Schnoor, Jerald L
AU  - Schnoor JL
FAU - Yoon, Jong Moon
AU  - Yoon JM
FAU - Van Aken, Benoit
AU  - Van Aken B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Int J Phytoremediation
JT  - International journal of phytoremediation
JID - 101136878
RN  - 0 (Acetonitriles)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Plant)
RN  - 0 (Explosive Agents)
RN  - 0 (RNA, Plant)
RN  - 0 (Triazines)
RN  - 118-96-7 (Trinitrotoluene)
RN  - W91SSV5831 (cyclonite)
RN  - Z072SB282N (acetonitrile)
SB  - IM
MH  - Acetonitriles/pharmacology
MH  - DNA Primers
MH  - DNA, Plant/genetics/isolation & purification
MH  - Explosive Agents/*pharmacology
MH  - Gene Expression Regulation, Plant/*drug effects
MH  - Kinetics
MH  - Polymerase Chain Reaction
MH  - Populus/drug effects/*genetics
MH  - RNA, Plant/genetics/isolation & purification
MH  - Triazines/*pharmacology
MH  - Trinitrotoluene/toxicity
EDAT- 2008/02/06 09:00
MHDA- 2008/02/27 09:00
CRDT- 2008/02/06 09:00
PHST- 2008/02/06 09:00 [pubmed]
PHST- 2008/02/27 09:00 [medline]
PHST- 2008/02/06 09:00 [entrez]
AID - 10.1080/15226510601139375 [doi]
PST - ppublish
SO  - Int J Phytoremediation. 2007 Jan-Feb;9(1):15-30. doi: 10.1080/15226510601139375.