PMID- 18247026
OWN - NLM
STAT- MEDLINE
DCOM- 20080710
LR  - 20191210
IS  - 0175-7598 (Print)
IS  - 0175-7598 (Linking)
VI  - 78
IP  - 5
DP  - 2008 Apr
TI  - Fluorescent in situ hybridization and flow cytometry as tools to evaluate the 
      treatments for the control of slime-forming enterobacteria in paper mills.
PG  - 889-97
LID - 10.1007/s00253-008-1369-6 [doi]
AB  - Slime formation is a serious problem nowadays in the paper industry. Some 
      enterobacteria are associated with the formation of slime deposits in paper and 
      board mills. Detection and characterization of slime forming bacteria, belonging 
      to the genus Enterobacter, Raoultella, and Klebsiella have been achieved by 
      fluorescence in situ hybridization (FISH), using one probe based on the 
      enterobacterial repetitive intergenic consensus sequence and other two rRNA 
      targeted oligonucleotide probes. The effects of three kinds of 
      antimicrobiological products (biocides, dispersants, and enzymes) on these 
      enterobacterial cells were analyzed by flow cytometry (FC). Biocides B: utrol 
      1009 and 1072 were the most effective microbiocides against all enterobacterial 
      cells analyzed, reaching 90% of dead bacteria after 24 h. However, the enzymatic 
      treatment (Buzyme) was not equally efficient on enterobacteria and its 
      microbiocide capacity varied depending on the type of microorganism. FISH and FC 
      were effective tools to detect important slime forming enterobacteria and to 
      select specific treatments to control microbial problems in the paper industry.
FAU - Torres, C Esperanza
AU  - Torres CE
AD  - Chemical Engineering Department, Faculty of Chemistry, Universidad Complutense, 
      Av. Complutense s/n, Madrid 28040, Spain.
FAU - Gibello, Alicia
AU  - Gibello A
FAU - Nande, Mar
AU  - Nande M
FAU - Martin, Margarita
AU  - Martin M
FAU - Blanco, Angeles
AU  - Blanco A
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080205
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal Spacer)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 0 (RNA, Ribosomal)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - DNA, Bacterial/genetics
MH  - DNA, Ribosomal Spacer/genetics
MH  - Enterobacteriaceae/drug effects/genetics/*isolation & purification/metabolism
MH  - Flow Cytometry/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Industrial Microbiology
MH  - Oligonucleotide Probes/genetics
MH  - *Paper
MH  - Polysaccharides, Bacterial/*metabolism
MH  - RNA, Ribosomal/genetics
MH  - Sensitivity and Specificity
EDAT- 2008/02/06 09:00
MHDA- 2008/07/11 09:00
CRDT- 2008/02/06 09:00
PHST- 2007/09/18 00:00 [received]
PHST- 2008/01/15 00:00 [accepted]
PHST- 2008/01/14 00:00 [revised]
PHST- 2008/02/06 09:00 [pubmed]
PHST- 2008/07/11 09:00 [medline]
PHST- 2008/02/06 09:00 [entrez]
AID - 10.1007/s00253-008-1369-6 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2008 Apr;78(5):889-97. doi: 10.1007/s00253-008-1369-6. 
      Epub 2008 Feb 5.