PMID- 18248528
OWN - NLM
STAT- MEDLINE
DCOM- 20080513
LR  - 20221207
IS  - 1365-3083 (Electronic)
IS  - 0300-9475 (Linking)
VI  - 67
IP  - 4
DP  - 2008 Apr
TI  - Immunostimulatory oligodeoxynucleotide containing TTTCGTTT motif from 
      Lactobacillus rhamnosus GG DNA potentially suppresses OVA-specific IgE production 
      in mice.
PG  - 370-6
LID - 10.1111/j.1365-3083.2008.02080.x [doi]
AB  - In a previous study of the immunoregulatory properties of commensal bacterial 
      DNA, we identified the strong immunostimulatory oligodeoxynucleotide (ISS-ODN) 
      ID35 in the genomic DNA of Lactobacillus rhamnosus GG (LGG). The observed effects 
      of ID35 are because of the unique TTTCGTTT motif located at the 5' end of the 
      ODN, which is different from the previously identified ISS motifs in humans and 
      mice. In the present study, we used an ovalbumin (OVA)-sensitized mouse model to 
      show that ID35 is a potent suppressor of antigen-specific immunoglobulin E (IgE) 
      production in vivo. This effect was toll-like receptor 9-dependent, as GpC 
      negID35 failed to suppress antigen-specific IgE production. ID35 activated the 
      specific subset of CD11c+CD8a+ dendritic cells, which are associated with 
      T-helper 1 (Th1)-type systemic responses, and effectively induced 
      interferon-gamma (IFN-gamma) production by CD4+ T cells in OVA-challenged mice. 
      These immunoregulatory effects of ID35 were comparable with those induced by the 
      murine prototype ODN 1826. Thus, ID35 is the first ISS-ODN with such a strong 
      immunostimulatory and IgE suppressor activity to be found in immunobiotic 
      bacterial DNA.
FAU - Iliev, I D
AU  - Iliev ID
AD  - Food Immunology Group, Laboratory of Animal Products Chemistry, Graduate School 
      of Agricultural Science, Tohoku University, Sendai, Japan.
FAU - Tohno, M
AU  - Tohno M
FAU - Kurosaki, D
AU  - Kurosaki D
FAU - Shimosato, T
AU  - Shimosato T
FAU - He, F
AU  - He F
FAU - Hosoda, M
AU  - Hosoda M
FAU - Saito, T
AU  - Saito T
FAU - Kitazawa, H
AU  - Kitazawa H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080201
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Allergens)
RN  - 0 (CD11c Antigen)
RN  - 0 (CD8 Antigens)
RN  - 0 (CD8 antigen, alpha chain)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Toll-Like Receptor 9)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Allergens/administration & dosage/*adverse effects
MH  - Amino Acid Motifs
MH  - Animals
MH  - Antibody Specificity
MH  - CD11c Antigen/metabolism
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8 Antigens/metabolism
MH  - CHO Cells
MH  - Cricetinae
MH  - Cricetulus
MH  - DNA, Bacterial/chemistry
MH  - Dendritic Cells/immunology/metabolism
MH  - Hypersensitivity/etiology/*immunology/metabolism
MH  - Immunoglobulin E/*immunology/metabolism
MH  - *Immunosuppression Therapy
MH  - Injections, Intraperitoneal
MH  - Interferon-gamma/biosynthesis/immunology
MH  - Lacticaseibacillus rhamnosus/chemistry/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Models, Animal
MH  - Oligodeoxyribonucleotides/administration & dosage/chemical 
      synthesis/genetics/*immunology
MH  - Ovalbumin/administration & dosage/*adverse effects/immunology
MH  - Probiotics/chemistry
MH  - Spleen/immunology
MH  - Toll-Like Receptor 9
EDAT- 2008/02/06 09:00
MHDA- 2008/05/14 09:00
CRDT- 2008/02/06 09:00
PHST- 2008/02/06 09:00 [pubmed]
PHST- 2008/05/14 09:00 [medline]
PHST- 2008/02/06 09:00 [entrez]
AID - SJI2080 [pii]
AID - 10.1111/j.1365-3083.2008.02080.x [doi]
PST - ppublish
SO  - Scand J Immunol. 2008 Apr;67(4):370-6. doi: 10.1111/j.1365-3083.2008.02080.x. 
      Epub 2008 Feb 1.