PMID- 18248690
OWN - NLM
STAT- MEDLINE
DCOM- 20081218
LR  - 20161020
IS  - 1461-1457 (Print)
IS  - 1461-1457 (Linking)
VI  - 11
IP  - 6
DP  - 2008 Sep
TI  - Effects of ethanol and 3,4-methylenedioxymethamphetamine (MDMA) alone or in 
      combination on spontaneous and evoked overflow of dopamine, serotonin and 
      acetylcholine in striatal slices of the rat brain.
PG  - 743-63
LID - 10.1017/S1461145708008481 [doi]
AB  - Ethanol (EtOH) potentiates the locomotor effects of 
      3,4-methylenedioxymetamphetamine (MDMA) in rats. This potentiation might involve 
      pharmacokinetic and/or pharmacodynamic mechanisms. We explored whether the latter 
      could be local. Using a slice superfusion approach, we assessed the effects of 
      MDMA (0.3, 3microm) and/or EtOH (2mm) on the spontaneous outflow and electrically 
      evoked release of serotonin (5-HT), dopamine (DA) and acetylcholine (ACh) in the 
      striatum, and for comparison, on 5-HT release in hippocampal and neocortical 
      tissue. MDMA and less effectively EtOH, augmented the outflow of 5-HT in all 
      regions. The electrically evoked 5-HT release was increased by MDMA at 3microm in 
      striatal slices only. With nomifensine throughout, EtOH significantly potentiated 
      the 0.3microm MDMA-induced outflow of 5-HT, but only in striatal slices. EtOH or 
      MDMA also enhanced the spontaneous outflow of DA, but MDMA reduced the 
      electrically evoked DA release. With fluvoxamine throughout superfusion, EtOH 
      potentiated the effect of MDMA on the spontaneous outflow of DA. Finally, 3microm 
      MDMA diminished the electrically evoked release of ACh, an effect involving 
      several receptors (D2, 5-HT2, NMDA, nicotinic, NK1), with some interactions with 
      EtOH. Among other results, we show for the first time a local synergistic 
      interaction of EtOH and MDMA on the spontaneous outflow of striatal DA and 5-HT, 
      which could be relevant to the EtOH-induced potentiation of hyperlocomotion in 
      MDMA-treated rats. These data do not preclude the contribution of other 
      pharmacodynamic and/or pharmacokinetic mechanisms in vivo but support the 
      hypothesis that EtOH may affect the abuse liability of MDMA.
FAU - Riegert, Celine
AU  - Riegert C
AD  - Institut fur Experimentelle und Klinische Pharmakologie und Toxikologie der 
      Universitat Freiburg, Neuropharmakologisches Labor, Freiburg, Germany.
FAU - Wedekind, Franziska
AU  - Wedekind F
FAU - Hamida, Sami Ben
AU  - Hamida SB
FAU - Rutz, Susanne
AU  - Rutz S
FAU - Rothmaier, Anna Katharina
AU  - Rothmaier AK
FAU - Jones, Byron C
AU  - Jones BC
FAU - Cassel, Jean-Christophe
AU  - Cassel JC
FAU - Jackisch, Rolf
AU  - Jackisch R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080204
PL  - England
TA  - Int J Neuropsychopharmacol
JT  - The international journal of neuropsychopharmacology
JID - 9815893
RN  - 0 (Central Nervous System Depressants)
RN  - 0 (Drug Combinations)
RN  - 0 (Serotonin Agents)
RN  - 10028-17-8 (Tritium)
RN  - 333DO1RDJY (Serotonin)
RN  - 3K9958V90M (Ethanol)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - N9YNS0M02X (Acetylcholine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Acetylcholine/*metabolism
MH  - Analysis of Variance
MH  - Animals
MH  - Behavior, Animal/drug effects
MH  - Central Nervous System Depressants/*pharmacology
MH  - Corpus Striatum/*drug effects/metabolism/radiation effects
MH  - Dopamine/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Drug Combinations
MH  - Electric Stimulation/methods
MH  - Ethanol/*pharmacology
MH  - In Vitro Techniques
MH  - Male
MH  - Motor Activity/drug effects
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Rats
MH  - Rats, Long-Evans
MH  - Serotonin/*metabolism
MH  - Serotonin Agents/*pharmacology
MH  - Tritium/metabolism
EDAT- 2008/02/06 09:00
MHDA- 2008/12/19 09:00
CRDT- 2008/02/06 09:00
PHST- 2008/02/06 09:00 [pubmed]
PHST- 2008/12/19 09:00 [medline]
PHST- 2008/02/06 09:00 [entrez]
AID - S1461145708008481 [pii]
AID - 10.1017/S1461145708008481 [doi]
PST - ppublish
SO  - Int J Neuropsychopharmacol. 2008 Sep;11(6):743-63. doi: 
      10.1017/S1461145708008481. Epub 2008 Feb 4.