PMID- 18249080
OWN - NLM
STAT- MEDLINE
DCOM- 20080522
LR  - 20191210
IS  - 0723-2020 (Print)
IS  - 0723-2020 (Linking)
VI  - 31
IP  - 1
DP  - 2008 Mar
TI  - Improved quantification of Dehalococcoides species by fluorescence in situ 
      hybridization and catalyzed reporter deposition.
PG  - 62-7
LID - 10.1016/j.syapm.2007.11.001 [doi]
AB  - Chlorinated ethenes contamination of soil and groundwater is a widespread problem 
      in most industrialized countries. To date, there is a general consensus in the 
      literature that members of the genus Dehalococcoides are required for complete 
      dechlorination of these compounds. The availability of specific identification 
      tools to track their distribution in the field is therefore a topic of particular 
      relevance in environmental studies. These microorganisms have been successfully 
      visualized by fluorescence in situ hybridization (FISH) in highly active 
      dechlorinating cultures. However, FISH detection of Dehalococcoides under low 
      activity conditions can be strongly hampered by their small cell size and low 
      ribosome content. In this study, catalyzed reporter deposition (CARD)-FISH was 
      employed as an alternative detection method. In a trichloroethene (TCE) 
      dechlorinating enrichment culture, CARD-FISH, using proteinase K as a 
      permeabilization pre-treatment, was found to be significantly superior to 
      conventional FISH in terms of both microscopic visualization and quantification 
      efficiency (about 30%). An application of this method on contaminated aquifer 
      samples is also presented and discussed.
FAU - Fazi, Stefano
AU  - Fazi S
AD  - Water Research Institute, IRSA-CNR, Via Reno 1, 00198 Rome, Italy.
FAU - Aulenta, Federico
AU  - Aulenta F
FAU - Majone, Mauro
AU  - Majone M
FAU - Rossetti, Simona
AU  - Rossetti S
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080130
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - EC 1.11.1.- (Horseradish Peroxidase)
RN  - EC 3.4.21.64 (Endopeptidase K)
RN  - WD06X94M2D (Vinyl Chloride)
SB  - IM
MH  - Chloroflexi/*genetics/*isolation & purification
MH  - Colony Count, Microbial/*methods
MH  - Endopeptidase K/metabolism
MH  - Horseradish Peroxidase/*metabolism
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microscopy
MH  - Permeability
MH  - Staining and Labeling/methods
MH  - Time Factors
MH  - Vinyl Chloride/metabolism
EDAT- 2008/02/06 09:00
MHDA- 2008/05/23 09:00
CRDT- 2008/02/06 09:00
PHST- 2007/07/11 00:00 [received]
PHST- 2007/11/16 00:00 [revised]
PHST- 2007/11/27 00:00 [accepted]
PHST- 2008/02/06 09:00 [pubmed]
PHST- 2008/05/23 09:00 [medline]
PHST- 2008/02/06 09:00 [entrez]
AID - S0723-2020(07)00134-8 [pii]
AID - 10.1016/j.syapm.2007.11.001 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2008 Mar;31(1):62-7. doi: 10.1016/j.syapm.2007.11.001. Epub 
      2008 Jan 30.