PMID- 18255080
OWN - NLM
STAT- MEDLINE
DCOM- 20080502
LR  - 20220331
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1185
IP  - 1
DP  - 2008 Mar 21
TI  - Determination of monosaccharide composition in plant fiber materials by capillary 
      zone electrophoresis.
PG  - 139-44
LID - 10.1016/j.chroma.2008.01.031 [doi]
AB  - The neutral sugar composition of acid hydrolyzed extracts of cellulose fiber 
      samples, i.e. oat spelt, wheat straw, thermomechanica pulp (TMP) made of spruce, 
      aspen stemwood, and bleached birch kraft pulp, was determined by a new capillary 
      zone electrophoresis (CZE) method employing an alkaline background electrolyte. 
      The method relies on in-capillary reaction and direct UV detection at wavelength 
      270 nm. Neutral carbohydrates D-(+)-galactose, D-(+)-glucose, L-rhamnose, 
      D-(+)-mannose, D-(-)-arabinose, and D-(+)-xylose were simultaneously separated. 
      The calibration plots were linear over a range from 10 to 150 mg/L for 
      D-(+)-galactose, L-rhamnose, D-(+)-mannose, and D-(-)-arabinose and from 50 to 
      400mg/L for D-(+)-glucose and D-(+)-xylose. Relative standard deviations (RSDs) 
      of peak areas during a 5-day analysis period varied from 3.3% for galactose to 
      11.8% for rhamnose. RSDs of migration times varied between 0.3 and 0.7%. The 
      detection limit (at S/N 3) was 5mg/L for each monosaccharide. The results 
      obtained by CZE agreed well with results obtained by high-performance 
      anion-exchange chromatography. Glucose and xylose were the two predominant 
      monosaccharides in the plants, except in the spruce TMP sample where glucose and 
      mannose dominated.
FAU - Rovio, Stella
AU  - Rovio S
AD  - Technical Research Centre of Finland, VTT, Espoo, Finland.
FAU - Simolin, Helena
AU  - Simolin H
FAU - Koljonen, Krista
AU  - Koljonen K
FAU - Siren, Heli
AU  - Siren H
LA  - eng
PT  - Journal Article
DEP - 20080119
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Carbohydrates)
RN  - 0 (Monosaccharides)
SB  - IM
MH  - Avena/chemistry
MH  - Carbohydrates/analysis
MH  - Electrophoresis, Capillary/*methods
MH  - Hydrolysis
MH  - Monosaccharides/*analysis
MH  - Picea/chemistry
MH  - Plants/*chemistry
MH  - Triticum/chemistry
EDAT- 2008/02/08 09:00
MHDA- 2008/05/03 09:00
CRDT- 2008/02/08 09:00
PHST- 2007/11/30 00:00 [received]
PHST- 2008/01/09 00:00 [revised]
PHST- 2008/01/15 00:00 [accepted]
PHST- 2008/02/08 09:00 [pubmed]
PHST- 2008/05/03 09:00 [medline]
PHST- 2008/02/08 09:00 [entrez]
AID - S0021-9673(08)00122-2 [pii]
AID - 10.1016/j.chroma.2008.01.031 [doi]
PST - ppublish
SO  - J Chromatogr A. 2008 Mar 21;1185(1):139-44. doi: 10.1016/j.chroma.2008.01.031. 
      Epub 2008 Jan 19.