PMID- 18256904
OWN - NLM
STAT- MEDLINE
DCOM- 20080731
LR  - 20181201
IS  - 1423-0127 (Electronic)
IS  - 1021-7770 (Linking)
VI  - 15
IP  - 3
DP  - 2008 May
TI  - In situ delivery of bone marrow cells and mesenchymal stem cells improves 
      cardiovascular function in hypertensive rats submitted to myocardial infarction.
PG  - 365-74
LID - 10.1007/s11373-008-9237-z [doi]
AB  - This work aimed to evaluate cardiac morphology/function and histological changes 
      induced by bone marrow cells (BMCs) and cultured mesenchymal stem cells (MSCs) 
      injected at the myocardium of spontaneously hypertensive rats (SHR) submitted to 
      surgical coronary occlusion. Female syngeneic adult SHR, submitted (MI) or not 
      (C) to coronary occlusion, were treated 24 h later with in situ injections of 
      normal medium (NM), or with MSCs (MSC) or BMCs (BM) from male rats. The animals 
      were evaluated after 1 and 30 days by echocardiography, histology of heart 
      sections and PCR for the Y chromosome. Improved ejection fraction and reduced 
      left ventricle infarcted area were observed in MSC rats as compared to the other 
      experimental groups. Treated groups had significantly reduced lesion tissue 
      score, increased capillary density and normal (not-atrophied) myocytes, as 
      compared to NM and C groups. The survival rate was higher in C, NM and MSC groups 
      as compared to MI and BM groups. In situ injection of both MSCs and BMCs resulted 
      in improved cardiac morphology, in a more physiological model of myocardial 
      infarction represented by surgical coronary occlusion of spontaneously 
      hypertensive rats. Only treatment with MSCs, however, ameliorated left ventricle 
      dysfunction, suggesting a positive role of these cells in heart remodeling in 
      infarcted hypertensive subjects.
FAU - de Macedo Braga, Luisa Maria Gomes
AU  - de Macedo Braga LM
AD  - Department of Genetics, Universidade Federal do Rio Grande do Sul, Av Bento 
      Goncalves 9500, 91501-970, Porto Alegre, RS, Brazil.
FAU - Lacchini, Silvia
AU  - Lacchini S
FAU - Schaan, Beatriz D'Agord
AU  - Schaan BD
FAU - Rodrigues, Bruno
AU  - Rodrigues B
FAU - Rosa, Kaleizu
AU  - Rosa K
FAU - De Angelis, Katia
AU  - De Angelis K
FAU - Borges, Luciano Figueiredo
AU  - Borges LF
FAU - Irigoyen, Maria Claudia
AU  - Irigoyen MC
FAU - Nardi, Nance Beyer
AU  - Nardi NB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080207
PL  - England
TA  - J Biomed Sci
JT  - Journal of biomedical science
JID - 9421567
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Bone Marrow Cells/*cytology
MH  - Cardiovascular System/physiopathology
MH  - *Cell Transplantation
MH  - DNA Primers
MH  - Disease Models, Animal
MH  - Echocardiography
MH  - Female
MH  - Hypertension/*surgery
MH  - Immunophenotyping
MH  - Mesenchymal Stem Cells/*cytology
MH  - Myocardial Infarction/diagnostic imaging/physiopathology/*surgery
MH  - Polymerase Chain Reaction
MH  - Rats
MH  - Rats, Inbred SHR
EDAT- 2008/02/08 09:00
MHDA- 2008/08/01 09:00
CRDT- 2008/02/08 09:00
PHST- 2007/08/24 00:00 [received]
PHST- 2008/01/14 00:00 [accepted]
PHST- 2008/02/08 09:00 [pubmed]
PHST- 2008/08/01 09:00 [medline]
PHST- 2008/02/08 09:00 [entrez]
AID - 10.1007/s11373-008-9237-z [doi]
PST - ppublish
SO  - J Biomed Sci. 2008 May;15(3):365-74. doi: 10.1007/s11373-008-9237-z. Epub 2008 
      Feb 7.