PMID- 18257270
OWN - NLM
STAT- MEDLINE
DCOM- 20090226
LR  - 20141120
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 32
IP  - 22
DP  - 2007 Nov
TI  - [Effect of Tongxinluo superfine on experimental anginal model (contraction of 
      collaterals) in rat with endothelial dysfunction].
PG  - 2404-8, 2426
AB  - OBJECTIVE: To study the effect of Tongxinluo superfine (TXL) on experimental 
      anginal model induced by Arginine Vasopressin in rats with endothelial 
      dysfunction. METHOD: First, the endothelial dysfunction rat model was made by 
      methionine-induced hyperhomocysteinemia (HHcy). The thoracic aorta were excised, 
      and acetylcholine (Ach)-induced endothelium dependent relaxation and sodium 
      nitroprusside (SNP) induced endothelium-independent relaxation were measured. 
      Total plasma homocysteine (Hcy) concentrations were measured with automated 
      fluorescence polarization immunoassay (FPIA). Enzyme-linked immunosorbent assay 
      (ELISA) was used to detect plasma von Willebrand factor (vWF) level. Plasma 
      nitric oxide (NO) contents were assayed by method of nitrate reductase. Then, the 
      rat model of collaterals contraction (model group) was established by AVP 
      intravenous injection in rats with endothelial dysfunction and the S wave change 
      (DeltaS) and T wave depression in Lead II ECG were used as the index of angina 
      severity. The nitric oxide (NO) contents in serum and the expression of 
      myocardium eNOS mRNA were measured. RESULT: Ach (0. 1-1000 nmol L(-1))-induced 
      endothelium dependent relaxation (EDR) of aortic rings was significantly 
      decreased in HHcy group. The endothelium-independent relaxation induced by SNP 
      (0.001-10 micromol L(-1)) was not significantly different between the two groups. 
      Plasma homocysteine concentrations and vWF levels in rats treated with methionine 
      were higher than those of control group, while NO contents were significantly 
      decreased in HHcy group compared with control. The results showed that 
      L-methionine intake induced hyperhomocysteinemia in rats. Impaired EDR, increased 
      vWF and decreased NO suggested the exist of endothelial dysfunction. DeltaS of 
      model group increased from 1 min to 5 min and T wave of model group depressed at 
      2 min compared with that of control after the administration of vasopressin (0.5 
      U kg(-1)). The intragastric administration of TXL inhibited vasopressin-induced S 
      wave change at 4 min and 5 min and T wave depression from 30 s to 3 min after AVP 
      injection. The NO contents in serum and the expression of myocardium eNOS mRNA of 
      TXL group were increased compared with model group. CONCLUSION: Experimental 
      angina induced by AVP injection is more severe in rats with endothelial 
      dysfunction. Tongxinluo Superfine can protect against collaterals contraction in 
      rats maybe by increasing the NO contents in serum and the expression of 
      myocardium eNOS mRNA.
FAU - Han, Yu-lian
AU  - Han YL
AD  - Department of Pathophysiology, Zhongshan School of Medicine, Guangzhou 510080, 
      China.
FAU - Cheng, Chao
AU  - Cheng C
FAU - Tan, Hong-mei
AU  - Tan HM
FAU - Wu, Wei-kang
AU  - Wu WK
FAU - Wu, Yi-ling
AU  - Wu YL
FAU - Sun, Hui-lan
AU  - Sun HL
FAU - Sun, Juan
AU  - Sun J
FAU - Chen, Jun-lin
AU  - Chen JL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Drug Combinations)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vasodilator Agents)
RN  - 0 (tongxinluo)
RN  - 0 (von Willebrand Factor)
RN  - 169D1260KM (Nitroprusside)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Aorta, Thoracic/drug effects/physiopathology
MH  - Drug Combinations
MH  - Drugs, Chinese Herbal/administration & dosage/isolation & 
      purification/*pharmacology
MH  - Electrocardiography
MH  - Endothelium, Vascular/*drug effects/physiopathology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Hyperhomocysteinemia/metabolism/physiopathology
MH  - In Vitro Techniques
MH  - Male
MH  - Myocardial Ischemia/blood/genetics/*physiopathology
MH  - Myocardium/metabolism/pathology
MH  - Nitric Oxide/blood
MH  - Nitric Oxide Synthase Type III/biosynthesis/genetics
MH  - Nitroprusside/pharmacology
MH  - Plants, Medicinal/chemistry
MH  - RNA, Messenger/genetics/metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Vasodilation/drug effects
MH  - Vasodilator Agents/pharmacology
MH  - von Willebrand Factor/metabolism
EDAT- 2008/02/09 09:00
MHDA- 2009/02/27 09:00
CRDT- 2008/02/09 09:00
PHST- 2008/02/09 09:00 [pubmed]
PHST- 2009/02/27 09:00 [medline]
PHST- 2008/02/09 09:00 [entrez]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2007 Nov;32(22):2404-8, 2426.