PMID- 18257297
OWN - NLM
STAT- MEDLINE
DCOM- 20100615
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 25
IP  - 6
DP  - 2007 Nov
TI  - [Determination of Jingzhaotoxin-I phospholipid membrane-binding activities by 
      high performance liquid chromatography with fluorescence detection].
PG  - 825-9
AB  - Jingzhaotoxin-I (JZTX-I), a 33-residue polypeptide with three disulfide bonds, 
      was a novel spider neurotoxin preferentially inhibiting cardiac sodium channel 
      inactivation. Its activities of phospholipid membrane-binding were studied by a 
      combination of reversed-phase high performance liquid chromatography (HPLC) and 
      fluorescence spectroscopy. Small unilamellar vesicles binding assays showed that 
      the partitioning of JZTX-I into lipid bilayer did not require negatively charged 
      phospholipids. Further, JZTX-I also exhibited a blue shift of 6.4 nm or 4.7 nm as 
      well as red-edge excitation shift of 7.4 nm or 8.0 nm in the presence of 75% 
      1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE )/25% 
      1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (POPG) 
      or 100% 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) vesicles 
      respectively, suggesting that some tryptophan residues on the hydrophobic surface 
      of the toxin were located within a motion restricted membrane interfacial region. 
      Fluorescence quenching experiments suggested that some tryptophan residues of 
      JZTX-I were positioned within the membrane and protected from aqueous quenching 
      agents. These findings should provide further insight into the molecular 
      mechanism of the channel gating of JZTX-I.
FAU - Zeng, Xiongzhi
AU  - Zeng X
AD  - The Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of 
      Education, Hunan Normal University, Changsha 410081, China. 
      xiongzhizeng@yahoo.com.cn
FAU - Pi, Jianhui
AU  - Pi J
FAU - Liang, Songping
AU  - Liang S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Lipid Bilayers)
RN  - 0 (Peptides)
RN  - 0 (Phospholipids)
RN  - 0 (Spider Venoms)
RN  - 0 (jingzhaotoxin-I, Chilobrachys jingzhao)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Lipid Bilayers/*chemistry
MH  - Peptides/*chemistry
MH  - Phospholipids/*chemistry
MH  - Protein Binding
MH  - Spectrometry, Fluorescence/*methods
MH  - Spider Venoms/*chemistry
EDAT- 2008/02/09 09:00
MHDA- 2010/06/16 06:00
CRDT- 2008/02/09 09:00
PHST- 2008/02/09 09:00 [pubmed]
PHST- 2010/06/16 06:00 [medline]
PHST- 2008/02/09 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2007 Nov;25(6):825-9.