PMID- 18261278
OWN - NLM
STAT- MEDLINE
DCOM- 20080818
LR  - 20140226
IS  - 0578-1426 (Print)
IS  - 0578-1426 (Linking)
VI  - 46
IP  - 11
DP  - 2007 Nov
TI  - [Effects of house dust mite allergen DNA vaccines on the allergic airway 
      inflammation in murine models].
PG  - 930-3
AB  - OBJECTIVE: To investigate the effects of allergen DNA vaccines on the airway 
      allergic inflammation in C57BL/6 mouse models sensitized and challenged with 
      house dust mite extracts. METHODS: C57BL/6 mice were sensitized and challenged 
      with house dust mite extract (HDME). After vaccinated with Der p1 or Der p2 DNA 
      vaccine or both, the mice were rechallenged with HDME. All mice underwent 
      pulmonary lavage in 24h after the last time of rechallenge, and the pathological 
      manifestations of the lung, cell counts and differentials, and IL-4, IL-5, IL-10, 
      IFNgamma, IgE and IgG1 in broncho-alveolar fluid (BALF) and serum were detected. 
      RESULTS: Airway eosinophilic inflammation was evident in mice 
      sensitized/challenged with HDME. Total cell count, eosinophil count and the 
      levels of IL-4, IL-5, IgE and IgG1 in BALF increased, and IL-10 decreased 
      significantly, while the IFNgamma level showed no change, as compared with the 
      control group. In the Der p1 group, the Der p2 group, and the Der p1 and p2 
      group, the infiltration of inflammatory cells and the levels of IL-4, IL-5, IgE 
      and IgG1 decreased; the Der p1 and p2 group showed no significant difference 
      compared with the control group. The effect was more significant in the Der p1 
      and p2 group compared to the Der p1 group and the Der p2. IFNgamma level showed 
      no significant change in three DNA vaccine groups. CONCLUSION: Der p1 and p2 DNA 
      vaccine, Der p1 DNA vaccine, and Der p2 DNA vaccine could efficiently reverse 
      established allergic inflammation. Der p1 and p2 DNA vaccine was more effective 
      than Der p1 DNA vaccine and Der p2 DNA vaccine.
FAU - Bi, Yu-tian
AU  - Bi YT
AD  - Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical 
      University, Chongqing 400037, China.
FAU - Wu, Kui
AU  - Wu K
FAU - Wang, Yan
AU  - Wang Y
FAU - Zhuo, Wen-lei
AU  - Zhuo WL
FAU - Wang, Chang-zheng
AU  - Wang CZ
FAU - Qian, Gui-sheng
AU  - Qian GS
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Nei Ke Za Zhi
JT  - Zhonghua nei ke za zhi
JID - 16210490R
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukin-5)
RN  - 0 (Vaccines, DNA)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Animals
MH  - Antigens, Dermatophagoides/*immunology
MH  - Bronchial Provocation Tests
MH  - Hypersensitivity/blood/*drug therapy/pathology
MH  - Immunoglobulin G/blood
MH  - Inflammation
MH  - Interleukin-10/blood
MH  - Interleukin-4/blood
MH  - Interleukin-5/blood
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pneumonia/blood/drug therapy/pathology
MH  - Pyroglyphidae/*immunology
MH  - Vaccines, DNA/immunology/*therapeutic use
EDAT- 2008/02/12 09:00
MHDA- 2008/08/19 09:00
CRDT- 2008/02/12 09:00
PHST- 2008/02/12 09:00 [pubmed]
PHST- 2008/08/19 09:00 [medline]
PHST- 2008/02/12 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Nei Ke Za Zhi. 2007 Nov;46(11):930-3.