PMID- 18261936
OWN - NLM
STAT- MEDLINE
DCOM- 20081204
LR  - 20220408
IS  - 1522-9653 (Electronic)
IS  - 1063-4584 (Linking)
VI  - 16
IP  - 9
DP  - 2008 Sep
TI  - A new player in cartilage homeostasis: adiponectin induces nitric oxide synthase 
      type II and pro-inflammatory cytokines in chondrocytes.
PG  - 1101-9
LID - 10.1016/j.joca.2007.12.008 [doi]
AB  - OBJECTIVE: Recent studies revealed a close connection between adipose tissue, 
      adipokines and articular degenerative inflammatory diseases such as rheumatoid 
      arthritis (RA) and osteoarthritis (OA). The goal of this work was to investigate 
      the activity of adiponectin in human and murine chondrocytes and to study its 
      functional role in the modulation of nitric oxide synthase type II (NOS2). For 
      completeness, interleukin (IL)-6, IL-1beta, matrix metalloproteinase (MMP)-2, 
      MMP-3, MMP-9, tissue inhibitor of metalloproteinase (TIMP)-1, prostaglandin E2 
      (PGE2), leukotriene B4 (LTB4), tumor necrosis factor alpha (TNF)-alpha and 
      monocyte chemoattractant protein-1 (MCP-1) accumulation have been evaluated in 
      adiponectin-stimulated chondrocytes cell culture supernatants. METHODS: Murine 
      ATDC5 cell line, C28/I2, C20A4, TC28a2 human immortalized chondrocytes, and human 
      cultured chondrocytes were used. Nitrite accumulation was determined by Griess 
      reaction. Adiponectin receptors (AdipoRs) expression was evaluated by 
      immunofluorescence microscopy and confirmed by reverse transcriptase-polymerase 
      chain reaction. NOS2 expression was evaluated by Western blot analysis whereas 
      cytokines, prostanoids and metalloproteinases production was evaluated by 
      specific enzyme-linked immunosorbent assays. RESULTS: Human and murine 
      chondrocytes express functional AdipoRs. Adiponectin induces NOS2. This effect is 
      inhibited by aminoguanidine, dexamethasone and by a selective inhibitor of 
      phosphatidylinositol 3-kinase. In addition, adiponectin is able to increase IL-6, 
      MMP-3, MMP-9 and MCP-1 by murine cultured chondrocytes whereas it was unable to 
      modulate TNF-alpha, IL-1beta, MMP-2, TIMP-1, PGE2 and LTB4 release. CONCLUSIONS: 
      These results bind more closely the interactions between fat-derived adipokines 
      and articular inflammatory diseases, and suggest that adiponectin is a novel key 
      element in the maintenance of cartilage homeostasis which might be considered as 
      a potential therapeutical target in joint degenerative diseases.
FAU - Lago, R
AU  - Lago R
AD  - Santiago University Clinical Hospital, Research Laboratory 4, NeuroEndocrine 
      Interactions in Rheumatology and Inflammatory Diseases Laboratory, Santiago de 
      Compostela, Spain.
FAU - Gomez, R
AU  - Gomez R
FAU - Otero, M
AU  - Otero M
FAU - Lago, F
AU  - Lago F
FAU - Gallego, R
AU  - Gallego R
FAU - Dieguez, C
AU  - Dieguez C
FAU - Gomez-Reino, J J
AU  - Gomez-Reino JJ
FAU - Gualillo, O
AU  - Gualillo O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080207
PL  - England
TA  - Osteoarthritis Cartilage
JT  - Osteoarthritis and cartilage
JID - 9305697
RN  - 0 (Adiponectin)
RN  - 0 (Cytokines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Adiponectin/pharmacology
MH  - Adipose Tissue, White/*metabolism/physiology
MH  - Animals
MH  - Arthritis, Rheumatoid/metabolism/pathology
MH  - Cartilage, Articular/*metabolism/pathology
MH  - Chondrocytes/*metabolism/pathology
MH  - Cytokines/*metabolism
MH  - Homeostasis/physiology
MH  - Humans
MH  - Matrix Metalloproteinases/*metabolism
MH  - Mice
MH  - Nitric Oxide Synthase Type II/*metabolism
MH  - Osteoarthritis/metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2008/02/12 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/02/12 09:00
PHST- 2007/05/07 00:00 [received]
PHST- 2007/12/22 00:00 [accepted]
PHST- 2008/02/12 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/02/12 09:00 [entrez]
AID - S1063-4584(07)00401-3 [pii]
AID - 10.1016/j.joca.2007.12.008 [doi]
PST - ppublish
SO  - Osteoarthritis Cartilage. 2008 Sep;16(9):1101-9. doi: 10.1016/j.joca.2007.12.008. 
      Epub 2008 Feb 7.