PMID- 18262381
OWN - NLM
STAT- MEDLINE
DCOM- 20080729
LR  - 20080512
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 47
IP  - 2
DP  - 2008 Jun 9
TI  - Liquid chromatography-atmospheric pressure ionization electrospray mass 
      spectrometry determination of "hallucinogenic designer drugs" in urine of 
      consumers.
PG  - 335-42
LID - 10.1016/j.jpba.2007.12.039 [doi]
AB  - A procedure based on liquid chromatography-mass spectrometry (LC-MS) is described 
      for determination of 3,4-methylenedioxymethamphetamine (MDMA), 
      2,5-dimethoxy-4-methyl-phenethylamine (2C-D), 
      4-bromo-2,5-dimethoxy-beta-phenethylamine (2C-B), 
      1-(8-bromo-2,3,6,7-tetrahydrobenzo[1,2-b:4,5-b'] difuran-4-yl)-2-aminoethane 
      (2C-B-Fly), 4-ethylthio-2,5-dimethoxy-beta-phenethylamine (2C-T-2), 
      4-iodo-2,5-dimethoxy-beta-phenethylamine (2C-I), and 
      4-ethyl-2,5-dimethoxy-beta-phenethylamine (2C-E), 1-(m-chlorophenyl)piperazine 
      (m-CPP), 4-hydroxy-N,N-diisopropyltryptamine (4-OH-DIPT) and 
      4-acetoxy-N,N-diisopropyltryptamine (4-acetoxy-DIPT) in urine of consumers using 
      3,4 methylendioxypropylamphetamine (MDPA) as internal standard. Sample 
      preparation involved a solid-phase extraction procedure at pH 6 of both 
      non-hydrolyzed and enzymatically hydrolyzed urine samples. Chromatography was 
      performed on a C(18) reversed-phase column using a linear gradient of 10mM 
      ammonium bicarbonate, pH 7.3 and acetonitrile as a mobile phase. Separated 
      analytes were determined in LC-MS single ion monitoring mode using an atmospheric 
      pressure ionization-electrospray ionization (ESI) interface. The assay was tested 
      on urine samples from consumers of compounds under investigation (n=32). Limits 
      of quantification varied between 20 and 60 ng/mL for the different analytes under 
      investigation. Calibration curves were linear to 2000 ng/mL for all the 
      substances under investigation, with a minimum r(2)>0.99. At three concentrations 
      spanning the linear dynamic range of the assay, mean recoveries ranged between 
      55.4 and 95.6% for the different analytes. Higher analytes concentrations in 
      hydrolyzed samples showed the presence of conjugated compounds in urine.
FAU - Pichini, Simona
AU  - Pichini S
AD  - Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore 
      di Sanita, Rome, Italy. simona.pichini@iss.it
FAU - Pujadas, Mitona
AU  - Pujadas M
FAU - Marchei, Emilia
AU  - Marchei E
FAU - Pellegrini, Manuela
AU  - Pellegrini M
FAU - Fiz, Jimena
AU  - Fiz J
FAU - Pacifici, Roberta
AU  - Pacifici R
FAU - Zuccaro, Piergiorgio
AU  - Zuccaro P
FAU - Farre, Magi
AU  - Farre M
FAU - de la Torre, Rafael
AU  - de la Torre R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080104
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Designer Drugs)
RN  - 0 (Hallucinogens)
SB  - IM
MH  - Adult
MH  - *Atmospheric Pressure
MH  - Calibration
MH  - Chromatography, Liquid/*methods
MH  - Designer Drugs/*analysis/chemistry
MH  - Drug Stability
MH  - Female
MH  - Freezing
MH  - Hallucinogens/*analysis/urine
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Substance Abuse Detection/*methods
MH  - Time Factors
EDAT- 2008/02/12 09:00
MHDA- 2008/07/30 09:00
CRDT- 2008/02/12 09:00
PHST- 2007/10/09 00:00 [received]
PHST- 2007/12/20 00:00 [revised]
PHST- 2007/12/21 00:00 [accepted]
PHST- 2008/02/12 09:00 [pubmed]
PHST- 2008/07/30 09:00 [medline]
PHST- 2008/02/12 09:00 [entrez]
AID - S0731-7085(08)00003-4 [pii]
AID - 10.1016/j.jpba.2007.12.039 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2008 Jun 9;47(2):335-42. doi: 10.1016/j.jpba.2007.12.039. 
      Epub 2008 Jan 4.