PMID- 18265944
OWN - NLM
STAT- MEDLINE
DCOM- 20080604
LR  - 20211020
IS  - 0167-4412 (Print)
IS  - 0167-4412 (Linking)
VI  - 66
IP  - 6
DP  - 2008 Apr
TI  - Agrobacterium-mediated transformation of maize (Zea mays) with Cre-lox site 
      specific recombination cassettes in BIBAC vectors.
PG  - 587-98
LID - 10.1007/s11103-007-9276-2 [doi]
AB  - The Cre/loxP site-specific recombination system has been applied in various plant 
      species including maize (Zea mays) for marker gene removal, gene targeting, and 
      functional genomics. A BIBAC vector system was adapted for maize transformation 
      with a large fragment of genetic material including a herbicide resistance marker 
      gene, a 30 kb yeast genomic fragment as a marker for fluorescence in situ 
      hybridization (FISH), and a 35S-lox-cre recombination cassette. Seventy-five 
      transgenic lines were generated from Agrobacterium-mediated transformation of a 
      maize Hi II line with multiple B chromosomes. Eighty-four inserts have been 
      localized among all 10 A chromosome pairs by FISH using the yeast DNA probe 
      together with a karyotyping cocktail. No inserts were found on the B chromosomes; 
      thus a bias against the B chromosomes by the Agrobacterium-mediated 
      transformation was revealed. The expression of a cre gene was confirmed in 68 of 
      the 75 transgenic lines by a reporter construct for cre/lox mediated 
      recombination. The placement of the cre/lox site-specific recombination system in 
      many locations in the maize genome will be valuable materials for gene targeting 
      and chromosome engineering.
FAU - Vega, Juan M
AU  - Vega JM
AD  - Division of Biological Sciences, University of Missouri, Tucker Hall, Columbia, 
      MO 65211, USA.
FAU - Yu, Weichang
AU  - Yu W
FAU - Han, Fangpu
AU  - Han F
FAU - Kato, Akio
AU  - Kato A
FAU - Peters, Eric M
AU  - Peters EM
FAU - Zhang, Zhanyuan J
AU  - Zhang ZJ
FAU - Birchler, James A
AU  - Birchler JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20080212
PL  - Netherlands
TA  - Plant Mol Biol
JT  - Plant molecular biology
JID - 9106343
SB  - IM
MH  - Blotting, Southern
MH  - Chromosomes, Plant/genetics
MH  - Genetic Vectors/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Models, Genetic
MH  - Plants, Genetically Modified
MH  - Recombination, Genetic/*genetics
MH  - Rhizobium/*genetics
MH  - Transformation, Genetic
MH  - Transgenes/genetics
MH  - Zea mays/*genetics
EDAT- 2008/02/13 09:00
MHDA- 2008/06/05 09:00
CRDT- 2008/02/13 09:00
PHST- 2007/10/21 00:00 [received]
PHST- 2007/12/17 00:00 [accepted]
PHST- 2008/02/13 09:00 [pubmed]
PHST- 2008/06/05 09:00 [medline]
PHST- 2008/02/13 09:00 [entrez]
AID - 10.1007/s11103-007-9276-2 [doi]
PST - ppublish
SO  - Plant Mol Biol. 2008 Apr;66(6):587-98. doi: 10.1007/s11103-007-9276-2. Epub 2008 
      Feb 12.