PMID- 18266902
OWN - NLM
STAT- MEDLINE
DCOM- 20081027
LR  - 20081121
IS  - 1365-3040 (Electronic)
IS  - 0140-7791 (Linking)
VI  - 31
IP  - 6
DP  - 2008 Jun
TI  - Transcriptome profiling of grapefruit flavedo following exposure to low 
      temperature and conditioning treatments uncovers principal molecular components 
      involved in chilling tolerance and susceptibility.
PG  - 752-68
LID - 10.1111/j.1365-3040.2008.01793.x [doi]
AB  - A pre-storage conditioning (CD) treatment of 16 degrees C for 7 d enhanced 
      chilling tolerance of grapefruit and reduced the development of chilling injuries 
      during storage at 5 degrees C. To gain a better understanding of the molecular 
      mechanisms involved in the responses of citrus fruit to low temperatures, we 
      performed genome-wide transcriptional profiling analysis of RNA isolated from 
      grapefruit flavedo using the newly developed Affymetrix Citrus GeneChip 
      microarray. Utilizing very restrictive cut-off criteria, including pair-wise 
      anova comparisons significantly different at P < or = 0.05 and induction or 
      repression of transcript levels by at least fourfold, we found that out of 30 171 
      probe sets on the microarray, 1345 probe sets were significantly affected by 
      chilling in both control and CD-treated fruits, 509 probe sets were affected by 
      chilling specifically in the CD-treated fruits, and 417 probe sets were 
      specifically expressed in chilling-sensitive control fruits. Overall, exposure to 
      chilling led to expression arrest of general cellular metabolic activity, 
      including concretive down-regulation of cell wall, pathogen defence, 
      photosynthesis, respiration, and protein, nucleic acid and secondary metabolism. 
      On the other hand, chilling enhanced adaptation processes that involve changes in 
      the expression of transcripts related to membranes, lipid, sterol and 
      carbohydrate metabolism, stress stimuli, hormone biosynthesis, and modifications 
      in DNA binding and transcription factors.
FAU - Maul, Pilar
AU  - Maul P
AD  - United States Department of Agriculture, US Horticultural Research Laboratory, 
      2001 S. Rock Road, Fort Pierce, FL 34945, USA.
FAU - McCollum, Gregory T
AU  - McCollum GT
FAU - Popp, Mick
AU  - Popp M
FAU - Guy, Charles L
AU  - Guy CL
FAU - Porat, Ron
AU  - Porat R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080205
PL  - United States
TA  - Plant Cell Environ
JT  - Plant, cell & environment
JID - 9309004
RN  - 0 (Plant Proteins)
SB  - IM
MH  - Adaptation, Physiological
MH  - Citrus paradisi/*genetics/*metabolism
MH  - *Cold Temperature
MH  - Down-Regulation
MH  - Fruit
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Plant/physiology
MH  - Lipid Metabolism
MH  - Plant Proteins/genetics/*metabolism
MH  - Time Factors
MH  - Up-Regulation
EDAT- 2008/02/13 09:00
MHDA- 2008/10/28 09:00
CRDT- 2008/02/13 09:00
PHST- 2008/02/13 09:00 [pubmed]
PHST- 2008/10/28 09:00 [medline]
PHST- 2008/02/13 09:00 [entrez]
AID - PCE1793 [pii]
AID - 10.1111/j.1365-3040.2008.01793.x [doi]
PST - ppublish
SO  - Plant Cell Environ. 2008 Jun;31(6):752-68. doi: 10.1111/j.1365-3040.2008.01793.x. 
      Epub 2008 Feb 5.