PMID- 18267101
OWN - NLM
STAT- MEDLINE
DCOM- 20080428
LR  - 20211020
IS  - 1096-0384 (Electronic)
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 472
IP  - 2
DP  - 2008 Apr 15
TI  - Identification of two epoxide hydrolases in Caenorhabditis elegans that 
      metabolize mammalian lipid signaling molecules.
PG  - 139-49
LID - 10.1016/j.abb.2008.01.016 [doi]
AB  - We have identified two genes in the genomic database for Caenorhabditis elegans 
      that code for proteins with significant sequence similarity to the mammalian 
      soluble epoxide hydrolase (sEH). The respective transcripts were cloned from a 
      mixed stage cDNA library from C. elegans. The corresponding proteins obtained 
      after recombinant expression in insect cells hydrolyzed standard epoxide 
      hydrolase substrates, including epoxyeicosatrienoic acids (EETs) and leukotoxins 
      (EpOMEs). The enzyme activity was inhibited by urea-based compounds originally 
      designed to inhibit the mammalian sEH. In vivo inhibition of the enzymes using 
      the most potent of these compounds resulted in elevated levels of the EpOMEs in 
      the nematode. These results suggest that the hydrolases are involved in the 
      metabolism of possible lipid signaling molecules in C. elegans.
FAU - Harris, Todd R
AU  - Harris TR
AD  - Department of Entomology and Cancer Research Center, University of California, 
      One Shields Avenue, Davis, CA 95616, USA.
FAU - Aronov, Pavel A
AU  - Aronov PA
FAU - Jones, Paul D
AU  - Jones PD
FAU - Tanaka, Hiromasa
AU  - Tanaka H
FAU - Arand, Michael
AU  - Arand M
FAU - Hammock, Bruce D
AU  - Hammock BD
LA  - eng
GR  - R37 ES002710-27/ES/NIEHS NIH HHS/United States
GR  - P42 ES004699/ES/NIEHS NIH HHS/United States
GR  - T32 ES007059-24/ES/NIEHS NIH HHS/United States
GR  - R37 ES02710/ES/NIEHS NIH HHS/United States
GR  - R37 ES002710/ES/NIEHS NIH HHS/United States
GR  - T32 ES007059/ES/NIEHS NIH HHS/United States
GR  - P42 ES004699-21/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080131
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (12,13-epoxy-9-octadecenoic acid)
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Exotoxins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Linoleic Acids)
RN  - 0 (Oleic Acids)
RN  - 0 (Recombinant Proteins)
RN  - 0 (leukotoxin)
RN  - 65167-83-1 (9,10-epoxy-12-octadecenoic acid)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Caenorhabditis elegans/*enzymology
MH  - Caenorhabditis elegans Proteins/antagonists & inhibitors/chemistry/*metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Epoxide Hydrolases/antagonists & inhibitors/chemistry/*metabolism
MH  - Exotoxins/chemistry
MH  - Fluorescent Dyes/chemistry
MH  - Gene Library
MH  - Linoleic Acids/chemistry/*metabolism
MH  - Molecular Sequence Data
MH  - Oleic Acids/chemistry/*metabolism
MH  - Recombinant Proteins/antagonists & inhibitors/chemistry
MH  - Substrate Specificity
PMC - PMC2435305
MID - NIHMS46147
EDAT- 2008/02/13 09:00
MHDA- 2008/04/29 09:00
PMCR- 2009/04/15
CRDT- 2008/02/13 09:00
PHST- 2007/09/11 00:00 [received]
PHST- 2008/01/12 00:00 [revised]
PHST- 2008/01/20 00:00 [accepted]
PHST- 2008/02/13 09:00 [pubmed]
PHST- 2008/04/29 09:00 [medline]
PHST- 2008/02/13 09:00 [entrez]
PHST- 2009/04/15 00:00 [pmc-release]
AID - S0003-9861(08)00039-8 [pii]
AID - 10.1016/j.abb.2008.01.016 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2008 Apr 15;472(2):139-49. doi: 10.1016/j.abb.2008.01.016. 
      Epub 2008 Jan 31.