PMID- 18267145
OWN - NLM
STAT- MEDLINE
DCOM- 20080807
LR  - 20080212
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 610
IP  - 1
DP  - 2008 Mar 3
TI  - Fungal pathogenic nucleic acid detection achieved with a microfluidic microarray 
      device.
PG  - 97-104
LID - 10.1016/j.aca.2007.12.048 [doi]
AB  - Detection of polymerase chain reaction (PCR) products obtained from cultured 
      greenhouse fungal pathogens, Botrytis cinerea and Didymella bryoniae has been 
      achieved using a previously developed microfluidic microarray assembly (MMA) 
      device. The flexible probe construction and rapid DNA detection resulted from the 
      use of centrifugal pumping in the steps of probe introduction and sample 
      delivery, respectively. The line arrays of the oligonucleotide probes were 
      "printed" on a CD-like glass chip using a polydimethylsiloxane (PDMS) polymer 
      plate with radial microfluidic channels, and the sample hybridizations were 
      conducted within the spiral channels on the second plate. The experimental 
      conditions of probe immobilization and sample hybridization were optimized, and 
      both complementary oligonucleotides and PCR products were tested. We were able to 
      achieve adequate fluorescent signals with a sample load as small as 0.5 nM (1 
      microL) for oligonucleotide samples; for PCR products, we achieved detection at 
      the level of 3 ng.
FAU - Wang, Lin
AU  - Wang L
AD  - Department of Chemistry, Simon Fraser University, 8888 University Drive, Burnaby, 
      V5A 1S6, BC, Canada.
FAU - Li, Paul C H
AU  - Li PC
FAU - Yu, Hua-Zhong
AU  - Yu HZ
FAU - Parameswaran, Ash M
AU  - Parameswaran AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080117
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Fungal)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers
MH  - DNA, Fungal/*analysis
MH  - Dimethylpolysiloxanes/chemistry
MH  - Fungi/*genetics
MH  - Microfluidics/*instrumentation
MH  - Nucleic Acid Hybridization
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Polymerase Chain Reaction
EDAT- 2008/02/13 09:00
MHDA- 2008/08/08 09:00
CRDT- 2008/02/13 09:00
PHST- 2007/09/21 00:00 [received]
PHST- 2007/12/18 00:00 [revised]
PHST- 2007/12/21 00:00 [accepted]
PHST- 2008/02/13 09:00 [pubmed]
PHST- 2008/08/08 09:00 [medline]
PHST- 2008/02/13 09:00 [entrez]
AID - S0003-2670(08)00041-X [pii]
AID - 10.1016/j.aca.2007.12.048 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2008 Mar 3;610(1):97-104. doi: 10.1016/j.aca.2007.12.048. Epub 
      2008 Jan 17.