PMID- 18267980
OWN - NLM
STAT- MEDLINE
DCOM- 20081215
LR  - 20111117
IS  - 1468-2060 (Electronic)
IS  - 0003-4967 (Linking)
VI  - 67
IP  - 12
DP  - 2008 Dec
TI  - Association between beta2 adrenergic receptor polymorphisms and rheumatoid 
      arthritis in conjunction with human leukocyte antigen (HLA)-DRB1 shared epitope.
PG  - 1759-64
LID - 10.1136/ard.2007.083782 [doi]
AB  - OBJECTIVE: In the present work, the frequency of inherited polymorphisms of the 
      beta2 adrenergic receptor (beta2AR) gene and their association with rheumatoid 
      arthritis (RA) as well as human leukocyte antigen (HLA)-DRB1 alleles was 
      examined. METHODS: An allele-specific polymerase chain reaction was used to 
      determine the common variants of the beta2AR at positions 16, 27 and 164 in 
      patients with RA (n=310) and ethnically matched healthy controls (n=305) from 
      Germany. HLA-DRB1 genotyping was performed by oligonucleotide hybridisation of 
      enzymatically amplified DNA allowing low-resolution HLA-DRB1 genotyping 
      comprising specificities DRB1*01 to DRB1*17. RESULTS: Arginine (Arg) at codon 16 
      was present in 278 patients with RA (89.7%) compared to 202 controls (66.2%; odds 
      ratio (OR) 4.43, 95% CI 2.81 to 7.02, p<0.001). Homozygosity for Arg16 was found 
      in 107 patients with RA (34.5%) compared to 14 controls (4.6%; OR 10.9, CI 5.9 to 
      20.5, p<0.001). Stratifying patients for their HLA-DR status revealed that 
      homozygosity for Arg16 exhibited the greatest risk for RA in combination with 
      HLA-DRB1*04 (OR 17.1, 95% CI 1.71 to 414.4, p=0.004). Interestingly, patients 
      with the Arg16 allele have a younger mean (SD) age at disease onset compared to 
      patients without Arg16 (46.1 (2.0) vs 53.1 (2.7) respectively, p<0.05). 
      Furthermore, 93.3% patients with homozygosity for Arg16 were positive for 
      anti-cyclic citrullinated peptide (CCP) antibodies vs 75% patients with 
      homozygosity for Gly16 (p<0.05). CONCLUSION: There was a highly significant 
      distortion between patients with RA and controls in the distribution of beta2AR 
      polymorphisms at codon 16, contributing (together with the HLA-DR alleles) to the 
      genetic background of RA.
FAU - Malysheva, O
AU  - Malysheva O
AD  - Medical Clinic IV, University Hospital, Liebigstrasse 22, 04103 Leipzig Germany. 
      Olga.Malysheva@medizin.uni-leipzig.de
FAU - Pierer, M
AU  - Pierer M
FAU - Wagner, U
AU  - Wagner U
FAU - Wahle, M
AU  - Wahle M
FAU - Wagner, U
AU  - Wagner U
FAU - Baerwald, C G
AU  - Baerwald CG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080211
PL  - England
TA  - Ann Rheum Dis
JT  - Annals of the rheumatic diseases
JID - 0372355
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (Receptors, Adrenergic, beta-2)
SB  - IM
MH  - Adult
MH  - Arthritis, Rheumatoid/*genetics
MH  - Case-Control Studies
MH  - Female
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB1 Chains
MH  - Heterozygote
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction/methods
MH  - *Polymorphism, Genetic
MH  - Receptors, Adrenergic, beta-2/*genetics
MH  - Severity of Illness Index
EDAT- 2008/02/13 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/02/13 09:00
PHST- 2008/02/13 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/02/13 09:00 [entrez]
AID - ard.2007.083782 [pii]
AID - 10.1136/ard.2007.083782 [doi]
PST - ppublish
SO  - Ann Rheum Dis. 2008 Dec;67(12):1759-64. doi: 10.1136/ard.2007.083782. Epub 2008 
      Feb 11.