PMID- 18268337
OWN - NLM
STAT- MEDLINE
DCOM- 20080313
LR  - 20211020
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 105
IP  - 7
DP  - 2008 Feb 19
TI  - Receptor determinants of zoonotic transmission of New World hemorrhagic fever 
      arenaviruses.
PG  - 2664-9
LID - 10.1073/pnas.0709254105 [doi]
AB  - Transferrin receptor 1 (TfR1) is a cellular receptor for the New World 
      hemorrhagic fever arenaviruses Machupo (MACV), Junin (JUNV), and Guanarito 
      (GTOV). Each of these viruses is specifically adapted to a distinct rodent host 
      species, but all cause human disease. Here we compare the ability of these 
      viruses to use various mammalian transferrin receptor 1 (TfR1) orthologs, 
      including those of the South American rodents that serve as reservoirs for MACV, 
      JUNV, and GTOV (Calomys callosus, Calomys musculinus, and Zygodontomys 
      brevicauda, respectively). Retroviruses pseudotyped with MACV and JUNV but not 
      GTOV glycoproteins (GPs) efficiently used C. callosus TfR1, whereas only JUNV GP 
      could use C. musculinus TfR1. All three viruses efficiently used Z. brevicauda 
      TfR1. TfR1 orthologs from related rodents, including house mouse (Mus musculus) 
      and rat (Rattus norvegicus), did not support entry of these viruses. In contrast, 
      these viruses efficiently used human and domestic cat TfR1 orthologs. We further 
      show that a local region of the human TfR1 apical domain, including tyrosine 211, 
      determined the efficiency with which MACV, JUNV, and GTOV used various TfR1 
      orthologs. Our data show that these New World arenaviruses are specifically 
      adapted to the TfR1 orthologs of their respective rodent hosts and identify key 
      commonalities between these orthologs and human TfR1 necessary for efficient 
      transmission of these viruses to humans.
FAU - Radoshitzky, Sheli R
AU  - Radoshitzky SR
AD  - Department of Microbiology and Molecular Genetics and New England Primate 
      Research Center, Harvard Medical School, Southborough, MA 01772, USA.
FAU - Kuhn, Jens H
AU  - Kuhn JH
FAU - Spiropoulou, Christina F
AU  - Spiropoulou CF
FAU - Albarino, Cesar G
AU  - Albarino CG
FAU - Nguyen, Dan P
AU  - Nguyen DP
FAU - Salazar-Bravo, Jorge
AU  - Salazar-Bravo J
FAU - Dorfman, Tatyana
AU  - Dorfman T
FAU - Lee, Amy S
AU  - Lee AS
FAU - Wang, Enxiu
AU  - Wang E
FAU - Ross, Susan R
AU  - Ross SR
FAU - Choe, Hyeryun
AU  - Choe H
FAU - Farzan, Michael
AU  - Farzan M
LA  - eng
SI  - GENBANK/EU164540
SI  - GENBANK/EU164541
SI  - GENBANK/EU340259
GR  - R01 AI074879/AI/NIAID NIH HHS/United States
GR  - R01 AI074879-01/AI/NIAID NIH HHS/United States
GR  - R01 AI074879-02/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20080211
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Receptors, Transferrin)
RN  - 42HK56048U (Tyrosine)
RN  - EC 3.6.5.2 (Monomeric GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Arenaviridae Infections/genetics/metabolism/*transmission
MH  - Arenaviruses, New World/*physiology
MH  - Binding Sites
MH  - Glycosylation
MH  - Humans
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Monomeric GTP-Binding Proteins/metabolism
MH  - Protein Binding
MH  - Protein Structure, Quaternary
MH  - Receptors, Transferrin/chemistry/classification/genetics/*metabolism
MH  - Sequence Alignment
MH  - Structural Homology, Protein
MH  - Tyrosine/genetics/metabolism
MH  - Virus Internalization
PMC - PMC2268193
COIS- The authors declare no conflict of interest.
EDAT- 2008/02/13 09:00
MHDA- 2008/03/14 09:00
PMCR- 2008/08/19
CRDT- 2008/02/13 09:00
PHST- 2008/02/13 09:00 [pubmed]
PHST- 2008/03/14 09:00 [medline]
PHST- 2008/02/13 09:00 [entrez]
PHST- 2008/08/19 00:00 [pmc-release]
AID - 0709254105 [pii]
AID - 9361 [pii]
AID - 10.1073/pnas.0709254105 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2664-9. doi: 
      10.1073/pnas.0709254105. Epub 2008 Feb 11.