PMID- 18268841
OWN - NLM
STAT- MEDLINE
DCOM- 20080313
LR  - 20190902
IS  - 0070-217X (Print)
IS  - 0070-217X (Linking)
VI  - 320
DP  - 2008
TI  - The mechanism of RNase III action: how dicer dices.
PG  - 99-116
AB  - Members of the Ribonuclease III (RNase III) family are double-stranded (ds) 
      RNA-specific endoribonucleases, characterized by a signature motif in their 
      active centers and a 2-nucleotide (nt) 3' overhang in their products. Dicer 
      functions as a dsRNA-processing enzyme, producing small interfering RNA (siRNA) 
      of approx. 24 nt in length (approx. 20-basepair RNA duplex with a 2-nt 3' 
      overhang on each end). Bacterial RNase III functions not only as a processing 
      enzyme, but also as a binding protein that binds dsRNA without cleaving it. As a 
      processing enzyme it produces siRNA-like RNA of approx. 13 nt in length (approx. 
      9-basepair duplex with a 2-nt 3' overhang on each end) as well as various types 
      of mature RNA. Dicer is structurally most complicated member of the family; 
      bacterial RNase III is comparatively much simpler. One structure is known for 
      Dicer in its RNA-free form (MacRae, Zhou, Li, Repic, Brooks, Cande, Adams, and 
      Doudna, Science 311:195-198); many structures are available for bacterial RNase 
      III, including the first catalytic complex of the entire family (Gan, Tropea, 
      Austin, Court, Waugh, and Ji, Cell 124:355-366). In light of the structural and 
      biochemical information on the RNase III proteins and the structure of a 
      non-Dicer PAZ (Piwi Argonaute Zwille) domain in complex with a 7-basepair RNA 
      duplex with a 2-nt 3' overhang on each end (Ma, Ye, and Patel, Nature 
      429:318-322), the structure and function of Dicer is being elucidated.
FAU - Ji, Xinhua
AU  - Ji X
AD  - Macromolecular Crystallography laboratory, National Cancer Institute, National 
      Institutes of Health, Frederick, MD 21702-1201, USA. jix@ncifcrf.gov
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Review
PL  - Germany
TA  - Curr Top Microbiol Immunol
JT  - Current topics in microbiology and immunology
JID - 0110513
RN  - 0 (RNA, Double-Stranded)
RN  - EC 3.1.26.3 (Ribonuclease III)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Crystallography, X-Ray
MH  - Humans
MH  - Protein Structure, Tertiary
MH  - *RNA Interference
MH  - RNA, Double-Stranded/metabolism
MH  - *Ribonuclease III/chemistry/classification/metabolism
RF  - 66
EDAT- 2008/02/14 09:00
MHDA- 2008/03/14 09:00
CRDT- 2008/02/14 09:00
PHST- 2008/02/14 09:00 [pubmed]
PHST- 2008/03/14 09:00 [medline]
PHST- 2008/02/14 09:00 [entrez]
AID - 10.1007/978-3-540-75157-1_5 [doi]
PST - ppublish
SO  - Curr Top Microbiol Immunol. 2008;320:99-116. doi: 10.1007/978-3-540-75157-1_5.