PMID- 18271260
OWN - NLM
STAT- MEDLINE
DCOM- 20090505
LR  - 20120409
IS  - 0001-6209 (Print)
IS  - 0001-6209 (Linking)
VI  - 47
IP  - 6
DP  - 2007 Dec
TI  - [Quantitative use of fluorescence in situ hybridization to detect syntrophic 
      acetogenic bacteria in anaerobic environmental samples].
PG  - 1038-43
AB  - Syntrophic acetogenic bacteria, an important functional one in anaerobic 
      habitats, were detected and counted by fluorescence in situ hybridization (FISH) 
      technology by using 16S rRNA-based oligonucleotide probes. For enumeration and 
      quantification of the targeted bacteria, an attempt was made to optimize the 
      hybridization conditions. The optimum conditions are as follows: a fixation time 
      of 19h, a dehydrated time of 5 min, and a formamide concentration of 55% in 
      hybridized solution. The abundance of syntrophic acetogenic bacteria of different 
      environmental samples were quantified by FISH and the results showed that Upflow 
      Anaerobic Sludge Reactor (UASB) treating STHZ high-concentration organic 
      wastewater and the digestive tract of some animals were the main habitats of 
      syntrophic acetogenic bacteria. The numbers of syntrophic acetogenic bacteria in 
      UASB and cattle manure were 1.70 x 10(9) cells/mL sample and 6.50 x 10(8) 
      cells/mL sample, respectively. Meanwhile, the sediments of rivers and lakes 
      existed less of the bacteria and the contents of them were just about 1.20 x 
      10(8) cells/mL sample in Taihu lake.
FAU - Li, Yan-Na
AU  - Li YN
AD  - Laboratory of Environmental Biotechnology, School of Biotechnology, Jiangnan 
      University, Wuxi 214122, China. liyanna-163@163.com
FAU - Xu, Ke-Wei
AU  - Xu KW
FAU - Du, Guo-Cheng
AU  - Du GC
FAU - Chen, Jian
AU  - Chen J
FAU - Liu, He
AU  - Liu H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Wei Sheng Wu Xue Bao
JT  - Wei sheng wu xue bao = Acta microbiologica Sinica
JID - 21610860R
RN  - 0 (Acetates)
RN  - 0 (Manure)
RN  - 0 (Sewage)
SB  - IM
MH  - Acetates/*metabolism
MH  - Bacteria, Anaerobic/*isolation & purification/metabolism
MH  - Geologic Sediments/microbiology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Manure/microbiology
MH  - Rivers/microbiology
MH  - Sewage/microbiology
EDAT- 2008/02/15 09:00
MHDA- 2009/05/06 09:00
CRDT- 2008/02/15 09:00
PHST- 2008/02/15 09:00 [pubmed]
PHST- 2009/05/06 09:00 [medline]
PHST- 2008/02/15 09:00 [entrez]
PST - ppublish
SO  - Wei Sheng Wu Xue Bao. 2007 Dec;47(6):1038-43.