PMID- 18271262
OWN - NLM
STAT- MEDLINE
DCOM- 20090505
LR  - 20120409
IS  - 0001-6209 (Print)
IS  - 0001-6209 (Linking)
VI  - 47
IP  - 6
DP  - 2007 Dec
TI  - [Identification and characterization of a novel antigenic protein of 
      Streptococcus suis type 2].
PG  - 1050-4
AB  - An immunogenic protein, named HM3, of Streptococcus suis type 2 HA9801 was 
      identified by using immunoproteomic assay. Some characters of this protein were 
      analyzed by several online bioinformatical tools, including BLAST, SinglP, HMMTOP 
      and PSORTb. The most homologous sequence of HM3 was extracellular solute-binding 
      protein (gi/69246104) of Enterococcus faecium. The predictions results showed 
      that HM3 contained Signal peptide and one transmembrane region, and 
      Non-Cytoplasmic Localization were also predicted. Partial gene of this protein 
      were amplified from the genome of HA9801 by PCR and inserted into expression 
      plasmid pET-32a (+) after double digested by BamH I and Sal I, then transformed 
      into BL21 (DE3) where they were induced to express by IPTG. After induced, there 
      was specific proteins band of approximately 45kDa on the SDS-PAGE gel. 
      Western-blotting showed that recombinant protein could react with immune serum of 
      HA9801 of SPF (Specefic pathogen Free) mini-swine. This protein could be taken as 
      vaccine candidate of SS2.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of 
      Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Wu, Zong-Fu
AU  - Wu ZF
FAU - Lu, Cheng-Ping
AU  - Lu CP
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Wei Sheng Wu Xue Bao
JT  - Wei sheng wu xue bao = Acta microbiologica Sinica
JID - 21610860R
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Bacterial/*analysis/immunology
MH  - Bacterial Proteins/*analysis/immunology
MH  - Computational Biology
MH  - Polymerase Chain Reaction
MH  - Proteomics
MH  - Recombinant Proteins/immunology
MH  - Streptococcus suis/*immunology
MH  - Swine
EDAT- 2008/02/15 09:00
MHDA- 2009/05/06 09:00
CRDT- 2008/02/15 09:00
PHST- 2008/02/15 09:00 [pubmed]
PHST- 2009/05/06 09:00 [medline]
PHST- 2008/02/15 09:00 [entrez]
PST - ppublish
SO  - Wei Sheng Wu Xue Bao. 2007 Dec;47(6):1050-4.