PMID- 18271969
OWN - NLM
STAT- MEDLINE
DCOM- 20080611
LR  - 20211020
IS  - 1465-542X (Electronic)
IS  - 1465-5411 (Print)
IS  - 1465-5411 (Linking)
VI  - 10
IP  - 1
DP  - 2008
TI  - Breast cancer stroma frequently recruits fetal derived cells during pregnancy.
PG  - R14
LID - 10.1186/bcr1860 [doi]
AB  - INTRODUCTION: Breast carcinomas associated with pregnancy display a high 
      frequency of inflammatory types, multifocal lesions and lymph node metastasis. 
      Because pregnancy results in transfer to mothers of foetal stem cells that can 
      migrate and differentiate into various tissues, we addressed the issue of whether 
      such cells are present in breast carcinoma associated with pregnancy. METHODS: We 
      analyzed women presenting with such tumours who were pregnant with male foetuses 
      using fluorescence in situ hybridization (FISH), targeting X and Y chromosomes. 
      The foetal cell phenotype was then determined by combining FISH and 
      immunohistochemistry with various antibodies. Statistical analysis was performed 
      using t-test or nonparametric Wilcoxon's test. RESULTS: We found that foetal 
      cells were present in nine out of 10 carcinomas, in contrast with none of four 
      benign mammary lesions (P < 0.05). Counting foetal and maternal cells showed that 
      the mean number of foetal cells per million maternal cells was 36 in breast 
      cancers and 0 in control samples (P < 0.01). By combining FISH and 
      immunolabelling, we found that foetal cells expressed mainly mesenchymal or, to a 
      lesser degree, epithelial or endothelial markers, but never leucocytes. 
      CONCLUSION: These findings demonstrate the frequent presence of foetal derived 
      cells essentially in tumour stroma. Given the role played by stroma in tumour 
      proliferation, these findings raise the issue of whether foetal cell can be 
      targeted to influence tumour behaviour.
FAU - Dubernard, Gil
AU  - Dubernard G
AD  - Universite Pierre et Marie Curie, Paris VI, EA 4053. Laboratoire de 
      Physiopathologie du developpement. 184 r Fbg St Antoine 75012 Paris, France. 
      gdubernard@yahoo.fr
FAU - Aractingi, Selim
AU  - Aractingi S
FAU - Oster, Michel
AU  - Oster M
FAU - Rouzier, Roman
AU  - Rouzier R
FAU - Mathieu, Marie-Christine
AU  - Mathieu MC
FAU - Uzan, Serge
AU  - Uzan S
FAU - Khosrotehrani, Kiarash
AU  - Khosrotehrani K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080213
PL  - England
TA  - Breast Cancer Res
JT  - Breast cancer research : BCR
JID - 100927353
SB  - IM
MH  - Adult
MH  - Breast Neoplasms/*pathology/*physiopathology
MH  - Carcinoma, Ductal, Breast/*pathology/*physiopathology
MH  - Female
MH  - Fetal Stem Cells/*physiology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Pregnancy
MH  - *Pregnancy Complications, Neoplastic
MH  - Retrospective Studies
PMC - PMC2374970
EDAT- 2008/02/15 09:00
MHDA- 2008/06/12 09:00
PMCR- 2008/02/13
CRDT- 2008/02/15 09:00
PHST- 2007/07/17 00:00 [received]
PHST- 2008/12/20 00:00 [revised]
PHST- 2008/02/13 00:00 [accepted]
PHST- 2008/02/15 09:00 [pubmed]
PHST- 2008/06/12 09:00 [medline]
PHST- 2008/02/15 09:00 [entrez]
PHST- 2008/02/13 00:00 [pmc-release]
AID - bcr1860 [pii]
AID - 10.1186/bcr1860 [doi]
PST - ppublish
SO  - Breast Cancer Res. 2008;10(1):R14. doi: 10.1186/bcr1860. Epub 2008 Feb 13.