PMID- 18272198
OWN - NLM
STAT- MEDLINE
DCOM- 20080908
LR  - 20131121
IS  - 0043-1354 (Print)
IS  - 0043-1354 (Linking)
VI  - 42
IP  - 10-11
DP  - 2008 May
TI  - Factors affecting the microbial populations at full-scale enhanced biological 
      phosphorus removal (EBPR) wastewater treatment plants in The Netherlands.
PG  - 2349-60
LID - 10.1016/j.watres.2008.01.001 [doi]
AB  - The influence of operating and environmental conditions on the microbial 
      populations of the enhanced biological phosphorus removal (EBPR) process at seven 
      full-scale municipal activated sludge wastewater treatment plants (WWTPs) in The 
      Netherlands was studied. Data from the selected WWTPs concerning process 
      configuration, operating and environmental conditions were compiled. The EBPR 
      activity from each plant was determined by execution of anaerobic-anoxic-aerobic 
      batch tests using fresh activated sludge. Fractions of Accumulibacter as 
      potential phosphorus accumulating organisms (PAO), and Competibacter, 
      Defluviicoccus-related microorganisms and Sphingomonas as potential glycogen 
      accumulating organisms (GAO) were quantified using fluorescence in situ 
      hybridization (FISH). The relationships among plant process configurations, 
      operating parameters, environmental conditions, EBPR activity and microbial 
      populations fractions were evaluated using a statistical approach. A well-defined 
      and operated denitrification stage and a higher mixed liquor pH value in the 
      anaerobic stage were positively correlated with the occurrence of Accumulibacter. 
      A well-defined denitrification stage also stimulated the development of 
      denitrifying PAO (DPAO). A positive correlation was observed between 
      Competibacter fractions and organic matter concentrations in the influent. 
      Nevertheless, Competibacter did not cause a major effect on the EBPR performance. 
      The observed Competibacter fractions were not in the range that would have led to 
      EBPR deterioration. Likely, the low average sewerage temperature (12+/-2 degrees 
      C) limited their proliferation. Defluviicoccus-related microorganisms were seen 
      only in negligible fractions in a few plants (<0.1% as EUB), whereas Sphingomonas 
      were not observed.
FAU - Lopez-Vazquez, Carlos M
AU  - Lopez-Vazquez CM
AD  - Department of Environmental Resources, UNESCO-IHE Institute for Water Education, 
      Wesvest 7, 2611 AX Delft, The Netherlands. c.lopezvazquez@unesco-ihe.org
FAU - Hooijmans, Christine M
AU  - Hooijmans CM
FAU - Brdjanovic, Damir
AU  - Brdjanovic D
FAU - Gijzen, Huub J
AU  - Gijzen HJ
FAU - van Loosdrecht, Mark C M
AU  - van Loosdrecht MC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080105
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (Sewage)
RN  - 27YLU75U4W (Phosphorus)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Aerobiosis
MH  - Anaerobiosis
MH  - Bacteria/*metabolism
MH  - Biodegradation, Environmental
MH  - Glycogen/metabolism
MH  - Netherlands
MH  - Phosphorus/*isolation & purification/metabolism
MH  - Sewage/microbiology
MH  - *Water Purification
EDAT- 2008/02/15 09:00
MHDA- 2008/09/09 09:00
CRDT- 2008/02/15 09:00
PHST- 2007/04/04 00:00 [received]
PHST- 2007/12/15 00:00 [revised]
PHST- 2008/01/02 00:00 [accepted]
PHST- 2008/02/15 09:00 [pubmed]
PHST- 2008/09/09 09:00 [medline]
PHST- 2008/02/15 09:00 [entrez]
AID - S0043-1354(08)00007-9 [pii]
AID - 10.1016/j.watres.2008.01.001 [doi]
PST - ppublish
SO  - Water Res. 2008 May;42(10-11):2349-60. doi: 10.1016/j.watres.2008.01.001. Epub 
      2008 Jan 5.