PMID- 18273837
OWN - NLM
STAT- MEDLINE
DCOM- 20080808
LR  - 20080310
IS  - 1098-2264 (Electronic)
IS  - 1045-2257 (Linking)
VI  - 47
IP  - 5
DP  - 2008 May
TI  - Association of chromosome band 8q22 copy number gain with high grade invasive 
      breast carcinomas by assessment of core needle biopsies.
PG  - 405-17
LID - 10.1002/gcc.20545 [doi]
AB  - Genetic analysis of breast tumor core needle biopsies may provide early 
      diagnostic information that is useful to direct subsequent clinical management. 
      We report metaphase comparative genomic hybridization (CGH) analysis of 42 core 
      needle biopsies prospectively sampled from invasive ductal breast carcinomas of 
      41 patients, and show that recurrent chromosomal copy number changes are 
      associated with histologically defined tumor features. As far as is known, this 
      is the first time CGH profiles from diagnostic breast tumor core needle biopsies 
      have been reported in association with pathological data in such detail. A 
      comparison of Grade 1 (n = 7), Grade 2 (n = 16), and Grade 3 tumors (n = 19) 
      revealed a chromosomal copy number gain involving 8q22 that was associated with 
      higher grade (1/7 vs. 16/19, respectively) and therefore altered cell 
      differentiation status. CGH results were validated using tumor touch imprints 
      prepared from a subgroup from the same sample set (n = 18) by fluorescence in 
      situ hybridization (FISH) and two probes selected from regions of interest within 
      8p21 and 8q22. Overall concordance between CGH and FISH for 8p21 and 8q22 
      imbalances was 9/18 (50%) and 12/18 (67%), respectively. When FISH and CGH data 
      were combined, and tumors classified according to better defined resected tumor 
      histology available for 34cases, 19/19 Grade 3 tumors showed 8q22 gain compared 
      with 0/6 Grade 1 tumors and 4/9 Grade 2 tumors. Further comprehensive studies are 
      required to verify the biological significance of this association and its 
      potential to facilitate early clinicopathological assessment of breast cancer. 
      This article contains Supplementary Material available at 
      http://www.interscience.wiley.com/jpages/1045-2257/suppmat.
CI  - (c) 2008 Wiley-Liss, Inc.
FAU - Walker, Logan C
AU  - Walker LC
AD  - Cancer Genetics Research Group, Department of Pathology, Christchurch School of 
      Medicine and Health Sciences, University of Otago, Christchurch, New Zealand.
FAU - Harris, Gavin C
AU  - Harris GC
FAU - Wells, J Elisabeth
AU  - Wells JE
FAU - Robinson, Bridget A
AU  - Robinson BA
FAU - Morris, Christine M
AU  - Morris CM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Biopsy, Needle
MH  - Breast Neoplasms/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 8
MH  - Cohort Studies
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Neoplasm Invasiveness
MH  - Nucleic Acid Hybridization
EDAT- 2008/02/15 09:00
MHDA- 2008/08/09 09:00
CRDT- 2008/02/15 09:00
PHST- 2008/02/15 09:00 [pubmed]
PHST- 2008/08/09 09:00 [medline]
PHST- 2008/02/15 09:00 [entrez]
AID - 10.1002/gcc.20545 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2008 May;47(5):405-17. doi: 10.1002/gcc.20545.