PMID- 18273885
OWN - NLM
STAT- MEDLINE
DCOM- 20080327
LR  - 20211020
IS  - 1096-9861 (Electronic)
IS  - 0021-9967 (Linking)
VI  - 507
IP  - 6
DP  - 2008 Apr 20
TI  - Aneuploid mosaicism in the developing and adult cerebellar cortex.
PG  - 1944-51
LID - 10.1002/cne.21648 [doi]
AB  - Neuroprogenitor cells (NPCs) in several telencephalic proliferative regions of 
      the mammalian brain, including the embryonic cerebral cortex and postnatal 
      subventricular zone (SVZ), display cell division "defects" in normal cells that 
      result in aneuploid adult progeny. Here, we identify the developing cerebellum as 
      a major, nontelencephalic proliferative region of the vertebrate central nervous 
      system (CNS) that also produces aneuploid NPCs and nonmitotic cells. Mitotic NPCs 
      assessed by metaphase chromosome analyses revealed that 15.3% and 20.8% of 
      cerebellar NPCs are aneuploid at P0 and P7, respectively. By using 
      immunofluorescent analysis of cerebellar NPCs, we show that chromosome 
      segregation defects contribute to the generation of cells with an aneuploid 
      genomic complement. Nonmitotic cells were assessed by fluorescence-activated cell 
      sorting (FACS) coupled with fluorescence in situ hybridization (FISH), which 
      revealed neuronal and nonneuronal aneuploid populations in both the adult mouse 
      and human cerebellum. Taken together, these results demonstrate that the 
      prevalence of neural aneuploidy includes nontelencephalic portions of the 
      neuraxis and suggest that the generation and maintenance of aneuploid cells is a 
      widespread, if not universal, property of central nervous system development and 
      organization.
CI  - (c) 2008 Wiley-Liss, Inc.
FAU - Westra, Jurjen W
AU  - Westra JW
AD  - Helen L. Dorris Child and Adolescent Neuropsychiatric Disorder Institute, The 
      Scripps Research Institute, La Jolla, California 92037, USA.
FAU - Peterson, Suzanne E
AU  - Peterson SE
FAU - Yung, Yun C
AU  - Yung YC
FAU - Mutoh, Tetsuji
AU  - Mutoh T
FAU - Barral, Serena
AU  - Barral S
FAU - Chun, Jerold
AU  - Chun J
LA  - eng
GR  - R21 MH076145/MH/NIMH NIH HHS/United States
GR  - MH076145/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
SB  - IM
MH  - Aging/genetics
MH  - *Aneuploidy
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Differentiation/genetics
MH  - Cell Proliferation
MH  - Cerebellar Cortex/cytology/*growth & development
MH  - Chromosome Segregation/genetics
MH  - Chromosomes/genetics
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Metaphase/genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mitosis/genetics
MH  - *Mosaicism
MH  - Neurons/cytology/*physiology
MH  - Stem Cells/cytology/*physiology
EDAT- 2008/02/15 09:00
MHDA- 2008/03/28 09:00
CRDT- 2008/02/15 09:00
PHST- 2008/02/15 09:00 [pubmed]
PHST- 2008/03/28 09:00 [medline]
PHST- 2008/02/15 09:00 [entrez]
AID - 10.1002/cne.21648 [doi]
PST - ppublish
SO  - J Comp Neurol. 2008 Apr 20;507(6):1944-51. doi: 10.1002/cne.21648.