PMID- 18274891
OWN - NLM
STAT- MEDLINE
DCOM- 20080624
LR  - 20211020
IS  - 0282-0080 (Print)
IS  - 0282-0080 (Linking)
VI  - 25
IP  - 3
DP  - 2008 Apr
TI  - Transcriptional regulation of the fucosyltransferase VI gene in hepatocellular 
      carcinoma cells.
PG  - 225-35
LID - 10.1007/s10719-008-9114-z [doi]
AB  - The alpha1,3-fucosyltransferase VI (FUT VI) protein is a key enzyme for synthesis 
      of sialyl Lewis X and Lewis X in epithelial cells. Despite its importance, how 
      FUT VI expression is regulated has not previously been elucidated. In this work, 
      we examined transcriptional regulation of the FUT VI gene in hepatocellular 
      carcinoma HepG2 cells. 5'-Rapid amplification of cDNA ends analysis revealed 
      transcription start sites of FUT VI in HepG2 cells at +65 and +278 nucleotides 
      (nt) downstream of the position registered in the Data Base of Human 
      Transcription Start Sites. We determined promoter regions for FUT VI in HepG2 
      cells using a luciferase reporter gene assay. The promoter activities of 
      constructs located 5'-upstream of the transcription start site decreased when the 
      -186 to -156 and -56 to -19 nt regions were deleted. Site-directed mutagenesis of 
      these regions revealed that two hepatocyte nuclear factor-4 alpha (HNF-4 alpha) 
      and one octamer binding transcription factor-1 (Oct-1) binding sites are 
      essential for FUT VI transcription. Furthermore, transient over-expression of 
      HNF-4 alpha but not Oct-1 enhanced both FUT VI promoter activities and FUT VI 
      mRNA levels in HuH-7 cells. These results suggest that two defined regions in the 
      5'-flanking region of the FUT VI transcription start site are critical for FUT VI 
      transcription in HepG2 cells.
FAU - Higai, Koji
AU  - Higai K
AD  - Department of Clinical Chemistry, School of Pharmaceutical Sciences, Toho 
      University, Miyama 2-2-1, Funabashi, Chiba, 274-8510, Japan. 
      koji@phar.toho-u.ac.jp
FAU - Miyazaki, Noriko
AU  - Miyazaki N
FAU - Azuma, Yutaro
AU  - Azuma Y
FAU - Matsumoto, Kojiro
AU  - Matsumoto K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080215
PL  - United States
TA  - Glycoconj J
JT  - Glycoconjugate journal
JID - 8603310
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HNF4A protein, human)
RN  - 0 (Hepatocyte Nuclear Factor 4)
RN  - 0 (OCA-S protein, human)
RN  - 0 (Transcription Factors)
RN  - EC 2.4.1.- (Fucosyltransferases)
RN  - EC 2.4.1.65 (FUT6 protein, human)
SB  - IM
MH  - 5' Flanking Region/genetics
MH  - Carcinoma, Hepatocellular/enzymology/*genetics
MH  - Cell Cycle Proteins/genetics
MH  - Cell Line, Tumor
MH  - DNA-Binding Proteins/genetics
MH  - Fucosyltransferases/*genetics
MH  - Gene Deletion
MH  - *Gene Expression Regulation, Enzymologic
MH  - *Gene Expression Regulation, Neoplastic
MH  - Hepatocyte Nuclear Factor 4/genetics
MH  - Humans
MH  - Liver Neoplasms/enzymology/*genetics
MH  - Mutagenesis, Site-Directed
MH  - Promoter Regions, Genetic/genetics
MH  - Transcription Factors/genetics
MH  - Transcription, Genetic/*genetics
EDAT- 2008/02/16 09:00
MHDA- 2008/06/25 09:00
CRDT- 2008/02/16 09:00
PHST- 2007/03/23 00:00 [received]
PHST- 2008/01/23 00:00 [accepted]
PHST- 2008/01/06 00:00 [revised]
PHST- 2008/02/16 09:00 [pubmed]
PHST- 2008/06/25 09:00 [medline]
PHST- 2008/02/16 09:00 [entrez]
AID - 10.1007/s10719-008-9114-z [doi]
PST - ppublish
SO  - Glycoconj J. 2008 Apr;25(3):225-35. doi: 10.1007/s10719-008-9114-z. Epub 2008 Feb 
      15.