PMID- 18276091
OWN - NLM
STAT- MEDLINE
DCOM- 20080818
LR  - 20181201
IS  - 0378-5173 (Print)
IS  - 0378-5173 (Linking)
VI  - 356
IP  - 1-2
DP  - 2008 May 22
TI  - Histological examination of PLGA nanospheres for intratracheal drug 
      administration.
PG  - 267-73
LID - 10.1016/j.ijpharm.2007.12.041 [doi]
AB  - Polylactide-glycolide (PLGA) nanospheres were reported as useful pulmonary drug 
      delivery carriers for improving the pharmacological effect of drug. This paper 
      describes the pathological and histological examinations of tissues after 
      intratracheal instillation of drug encapsulated PLGA nanospheres. After 
      intratracheally introducing FITC encapsulated PLGA nanospheres (dispersed in the 
      0.5 ml saline followed by mixing with an equal volume of air) to a rat, FITC was 
      found existing in the rat's lungs, liver, kidney, brain, spleen and pancreas as 
      demonstrated by immuno-histo-chemical staining with the dye. In this study, FITC 
      stayed in alveoli at least for 1.5h after the intratracheal administration of the 
      PLGA nanospheres, but the FITC almost disappeared 24h later. In addition, it was 
      found that the PLGA nanospheres were absorbed in the blood immediately (within 
      0.25 h after the intratracheal administration) through the type 1 alveolar 
      epithelium cell. Furthermore, the PLGA nanospheres were found resistant to uptake 
      by macrophages such as alveolus macrophages and kupffer cells. The results showed 
      that the possibility to induce tissue damage caused by the excessive immune 
      response from the deposition of PLGA nanospheres was very low, because the 
      nanospheres were not treated as foreign substances.
FAU - Hara, Kaori
AU  - Hara K
AD  - Hosokawa Powder Technology Research Institute, 1-9, Shoudai, Tajika, Hirakata, 
      Osaka 573-1132, Japan. khara@hmc.hosokawa.com
FAU - Tsujimoto, Hiroyuki
AU  - Tsujimoto H
FAU - Tsukada, Yusuke
AU  - Tsukada Y
FAU - Huang, C C
AU  - Huang CC
FAU - Kawashima, Yoshiaki
AU  - Kawashima Y
FAU - Tsutsumi, Masahiro
AU  - Tsutsumi M
LA  - eng
PT  - Journal Article
DEP - 20080108
PL  - Netherlands
TA  - Int J Pharm
JT  - International journal of pharmaceutics
JID - 7804127
RN  - 0 (Drug Carriers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Animals
MH  - Chemistry, Pharmaceutical
MH  - Drug Carriers/*chemistry
MH  - *Drug Delivery Systems
MH  - Fluorescein-5-isothiocyanate/*administration & dosage/pharmacokinetics
MH  - Immunohistochemistry
MH  - Kupffer Cells/metabolism
MH  - Lactic Acid/*chemistry
MH  - Macrophages, Alveolar/metabolism
MH  - Male
MH  - Nanospheres
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Pulmonary Alveoli/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - Tissue Distribution
EDAT- 2008/02/16 09:00
MHDA- 2008/08/19 09:00
CRDT- 2008/02/16 09:00
PHST- 2007/08/01 00:00 [received]
PHST- 2007/12/04 00:00 [revised]
PHST- 2007/12/19 00:00 [accepted]
PHST- 2008/02/16 09:00 [pubmed]
PHST- 2008/08/19 09:00 [medline]
PHST- 2008/02/16 09:00 [entrez]
AID - S0378-5173(07)01071-X [pii]
AID - 10.1016/j.ijpharm.2007.12.041 [doi]
PST - ppublish
SO  - Int J Pharm. 2008 May 22;356(1-2):267-73. doi: 10.1016/j.ijpharm.2007.12.041. 
      Epub 2008 Jan 8.