PMID- 18276953
OWN - NLM
STAT- MEDLINE
DCOM- 20080320
LR  - 20161124
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Linking)
VI  - 15
IP  - 2
DP  - 2008 Feb
TI  - Tumor necrosis factor-alpha converting enzyme in the human placenta throughout 
      gestation.
PG  - 195-209
LID - 10.1177/1933719107310709 [doi]
AB  - Ectodomain shedding of epidermal growth factor receptor ligands such as 
      transforming growth factor- alpha (TGF-alpha), heparin-binding epidermal growth 
      factor-like growth factor (HBEGF), and amphiregulin (AREG) is considered to be 
      important during implantation. Tumor necrosis factor-alpha converting enzyme 
      (TACE) has been suggested as the major sheddase for these molecules. The 
      objectives of this study are (1) to characterize the expression of TACE in the 
      human placenta throughout gestation; (2) to determine the association between the 
      expression of TACE with TGF-alpha, HBEGF, and AREG; (3) to ascertain whether TACE 
      mediates TGF-alpha, HBEGF, and AREG shedding; and (4) to examine the effect of 
      hypoxia on the expression of TACE. By analyzing a total of 55 villous samples 
      representing different gestational ages, the authors found that TACE was 
      continuously expressed in the placentas throughout gestation and that the levels 
      of TACE were positively correlated with the levels of TGF-alpha, HBEGF, and AREG. 
      Preadministration of a TACE inhibitor in villous explant cultures or transfection 
      of cytotrophoblastic cells with TACE-specific small interference RNA decreased 
      the shedding of HBEGF and AREG. Moreover, hypoxia (2% O(2)) caused an increase in 
      the levels of TACE mRNA and protein in villous explants and primary 
      cytotrophoblastic cells in vitro. These results indicate that oxygen regulates 
      the expression of TACE and that TACE may be important for placental development 
      during human pregnancy.
FAU - Hung, Tai-Ho
AU  - Hung TH
AD  - Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Taipei, 
      Taiwan.
FAU - Chen, Szu-Fu
AU  - Chen SF
FAU - Hsieh, Ching-Chang
AU  - Hsieh CC
FAU - Hsu, Jenn-Jeih
AU  - Hsu JJ
FAU - Li, Meng-Jen
AU  - Li MJ
FAU - Yeh, Yi-Lin
AU  - Yeh YL
FAU - Hsieh, T'sang-T'ang
AU  - Hsieh TT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (HBEGF protein, human)
RN  - 0 (Heparin-binding EGF-like Growth Factor)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.86 (ADAM17 Protein)
RN  - EC 3.4.24.86 (ADAM17 protein, human)
SB  - IM
MH  - ADAM Proteins/*biosynthesis
MH  - ADAM17 Protein
MH  - Amphiregulin
MH  - EGF Family of Proteins
MH  - Female
MH  - Glycoproteins/metabolism
MH  - Heparin-binding EGF-like Growth Factor
MH  - Humans
MH  - Hypoxia/metabolism
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Placenta/*enzymology/metabolism
MH  - Pregnancy/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2008/02/16 09:00
MHDA- 2008/03/21 09:00
CRDT- 2008/02/16 09:00
PHST- 2008/02/16 09:00 [pubmed]
PHST- 2008/03/21 09:00 [medline]
PHST- 2008/02/16 09:00 [entrez]
AID - 15/2/195 [pii]
AID - 10.1177/1933719107310709 [doi]
PST - ppublish
SO  - Reprod Sci. 2008 Feb;15(2):195-209. doi: 10.1177/1933719107310709.