PMID- 18279700
OWN - NLM
STAT- MEDLINE
DCOM- 20080328
LR  - 20131121
IS  - 0188-4409 (Print)
IS  - 0188-4409 (Linking)
VI  - 39
IP  - 3
DP  - 2008 Apr
TI  - Positive correlation of tumor necrosis factor-alpha early expression in 
      myocardium and ventricular arrhythmias in rats with acute myocardial infarction.
PG  - 285-91
LID - 10.1016/j.arcmed.2007.10.004 [doi]
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) is found to play important 
      roles in acute myocardial infarction (AMI). Ventricular arrhythmias arising from 
      AMI are leading causes of sudden cardiac death (SCD). We sought to clarify the 
      effect of TNF-alpha early expression on ventricular arrhythmias in rats with AMI 
      and its mechanism. METHODS: Rats with AMI were induced by left anterior 
      descending coronary branch ligation. The mRNA and protein levels of TNF-alpha in 
      myocardium were detected by real-time fluorescent quantitative PCR, Western 
      blotting and histochemistry. Meanwhile, electrocardiogram was recorded. Different 
      concentrations of TNF-alpha were added to isolated rat hearts in isolated heart 
      perfusions. Effect of TNF-alpha on intracellular Ca(2+) concentration was 
      detected by laser confocal technique. RESULTS: In AMI rats, mRNA and protein 
      levels of TNF-alpha were higher than control (p <0.05), and the occurrence time 
      of ventricular arrhythmias coincided with the secretion of TNF-alpha. TNF-alpha 
      may cause ventricular arrhythmias in isolated rat heart perfusion models. 
      Intracellular Ca(2+) intensity may quickly be increased by TNF-alpha. 
      CONCLUSIONS: Our results reveal the positive correlation between TNF-alpha early 
      expression and ventricular arrhythmias in rats with AMI. This effect may be 
      associated with the increased intracellular Ca(2+) intensity caused by TNF-alpha.
FAU - Xiao, Hua
AU  - Xiao H
AD  - Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, 
      Tong Ji Medical College, Hua Zhong University of Science and Technology, People's 
      Republic of China.
FAU - Chen, Zhijian
AU  - Chen Z
FAU - Liao, Yuhua
AU  - Liao Y
FAU - Cheng, Xiang
AU  - Cheng X
FAU - Liu, Kun
AU  - Liu K
FAU - Wang, Yanfu
AU  - Wang Y
FAU - Wang, Min
AU  - Wang M
FAU - Guo, Heping
AU  - Guo H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Med Res
JT  - Archives of medical research
JID - 9312706
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Arrhythmias, Cardiac/genetics/*metabolism/physiopathology
MH  - Calcium/metabolism
MH  - Gene Expression Regulation/*genetics
MH  - Immunohistochemistry
MH  - Myocardial Reperfusion Injury/genetics/*metabolism/physiopathology
MH  - Myocardium/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/genetics/*metabolism
EDAT- 2008/02/19 09:00
MHDA- 2008/03/29 09:00
CRDT- 2008/02/19 09:00
PHST- 2007/08/31 00:00 [received]
PHST- 2007/10/04 00:00 [accepted]
PHST- 2008/02/19 09:00 [pubmed]
PHST- 2008/03/29 09:00 [medline]
PHST- 2008/02/19 09:00 [entrez]
AID - S0188-4409(07)00360-8 [pii]
AID - 10.1016/j.arcmed.2007.10.004 [doi]
PST - ppublish
SO  - Arch Med Res. 2008 Apr;39(3):285-91. doi: 10.1016/j.arcmed.2007.10.004.