PMID- 18279951
OWN - NLM
STAT- MEDLINE
DCOM- 20080618
LR  - 20131121
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 29
IP  - 14
DP  - 2008 May
TI  - The effect of water-soluble chitosan on macrophage activation and the attenuation 
      of mite allergen-induced airway inflammation.
PG  - 2173-82
LID - 10.1016/j.biomaterials.2008.01.023 [doi]
AB  - Chitin and chitosan have versatile anti-tumor, anti-fungal, and antimicrobial 
      biological properties. Oral intakes and intranasal administration of chitin 
      attenuated allergen-induced airway inflammation in sensitized mice, which may be 
      due to its Th1 adjuvant properties. However, their mechanism of action is not 
      entirely clear. In this report, we demonstrate that water-soluble chitosan (WSC) 
      has specific immunomodulatory effects on dust mite allergen Dermatophagoides 
      farinae (Der f)-stimulated, monocyte-derived macrophages (MDM). These effects 
      include polarizing the cytokine balance towards Th1 cytokines, decreasing the 
      production of the inflammatory cytokines IL-6 and TNF-alpha, down-regulating CD44 
      and TLR4 receptor expression, and inhibiting T cell proliferation. Scanning 
      electron microscope (SEM) examination found that WSC reduced the rate of 
      pseudopodia formation in Der f-stimulated MDM from allergic asthma patients. The 
      effect of WSC on allergen-stimulated MDM may be mediated via inhibition of 
      PKCzeta phosphorylation and NF-kappaB pathway activation. In a murine model of 
      asthma, we found that intranasal application of WSC attenuates Der f-induced lung 
      inflammation by reducing infiltration of inflammatory cells, epithelial damage, 
      and goblet cell hyperplasia and markedly decreasing production of Arg I, iNOs, 
      and thymic stromal lymphopoietin (TSLP) in the bronchial epithelium. Therefore, 
      we believe that WSC may provide a new therapeutic modality for allergic asthma.
FAU - Chen, Chin-Lung
AU  - Chen CL
AD  - Institutes of Basic Medicine Sciences, College of Medicine, National Cheng Kung 
      University, Tainan, Taiwan.
FAU - Wang, Yu-Ming
AU  - Wang YM
FAU - Liu, Chia-Fang
AU  - Liu CF
FAU - Wang, Jiu-Yao
AU  - Wang JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080214
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Cytokines)
RN  - 059QF0KO0R (Water)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Animals
MH  - Antigens, Dermatophagoides
MH  - Asthma/*immunology/pathology
MH  - Bronchi/drug effects/immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chitosan/*pharmacology
MH  - Coculture Techniques
MH  - Cytokines/genetics/metabolism
MH  - Dermatophagoides farinae/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/drug effects/pathology
MH  - Female
MH  - Humans
MH  - Inflammation/immunology/metabolism
MH  - Macrophage Activation/*drug effects/immunology
MH  - Macrophages, Alveolar/*drug effects/immunology/ultrastructure
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Pseudopodia/drug effects
MH  - Solubility
MH  - T-Lymphocytes/physiology
MH  - Water/chemistry
EDAT- 2008/02/19 09:00
MHDA- 2008/06/19 09:00
CRDT- 2008/02/19 09:00
PHST- 2007/12/07 00:00 [received]
PHST- 2008/01/28 00:00 [accepted]
PHST- 2008/02/19 09:00 [pubmed]
PHST- 2008/06/19 09:00 [medline]
PHST- 2008/02/19 09:00 [entrez]
AID - S0142-9612(08)00064-1 [pii]
AID - 10.1016/j.biomaterials.2008.01.023 [doi]
PST - ppublish
SO  - Biomaterials. 2008 May;29(14):2173-82. doi: 10.1016/j.biomaterials.2008.01.023. 
      Epub 2008 Feb 14.