PMID- 18280126
OWN - NLM
STAT- MEDLINE
DCOM- 20080626
LR  - 20080305
IS  - 0953-7562 (Print)
IS  - 0953-7562 (Linking)
VI  - 112
IP  - Pt 2
DP  - 2008 Feb
TI  - 6-MSAS-like polyketide synthase genes occur in lichenized ascomycetes.
PG  - 289-96
LID - 10.1016/j.mycres.2007.08.023 [doi]
AB  - Lichenized and non-lichenized filamentous ascomycetes produce a great variety of 
      polyketide secondary metabolites. Some polyketide synthase (PKS) genes from 
      non-lichenized fungi have been characterized, but the function of PKS genes from 
      lichenized species remains unknown. Phylogenetic analysis of keto synthase (KS) 
      domains allows prediction of the presence or absence of particular domains in the 
      PKS gene. In the current study we screened genomic DNA from lichenized fungi for 
      the presence of non-reducing and 6-methylsalicylic acid synthase (6-MSAS)-type 
      PKS genes. We developed new degenerate primers in the acyl transferase (AT) 
      region to amplify a PKS fragment spanning most of the KS region, the entire 
      linker between KS and AT, and half of the AT region. Phylogenetic analysis shows 
      that lichenized taxa possess PKS genes of the 6-MSAS-type. The extended alignment 
      confirms overall phylogenetic relationships between fungal non-reducing, 
      6-MSAS-type and bacterial type I PKS genes.
FAU - Schmitt, Imke
AU  - Schmitt I
AD  - Department of Botany, The Field Museum, 1400 South Lake Shore Drive, Chicago, IL 
      60605, USA. schm2109@umn.edu
FAU - Kautz, Stefanie
AU  - Kautz S
FAU - Lumbsch, H Thorsten
AU  - Lumbsch HT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070929
PL  - England
TA  - Mycol Res
JT  - Mycological research
JID - 8913481
RN  - 0 (DNA, Fungal)
RN  - 0 (Fungal Proteins)
RN  - 0 (Multienzyme Complexes)
RN  - 79956-01-7 (Polyketide Synthases)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 6.- (6-methylsalicylic acid synthetase)
RN  - EC 6.- (Ligases)
SB  - IM
MH  - Acyltransferases/chemistry/*genetics
MH  - Amino Acid Sequence
MH  - Ascomycota/classification/*enzymology/genetics/metabolism
MH  - Bacteria/enzymology/genetics
MH  - DNA, Fungal/analysis/genetics
MH  - Fungal Proteins/chemistry/genetics
MH  - Genome, Fungal
MH  - Lichens/classification/*enzymology/genetics/metabolism
MH  - Ligases/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Multienzyme Complexes/chemistry/*genetics
MH  - Oxidoreductases/chemistry/*genetics
MH  - Phylogeny
MH  - Polyketide Synthases/chemistry/*genetics
MH  - Protein Structure, Tertiary
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
EDAT- 2008/02/19 09:00
MHDA- 2008/06/27 09:00
CRDT- 2008/02/19 09:00
PHST- 2006/12/20 00:00 [received]
PHST- 2007/07/03 00:00 [revised]
PHST- 2007/08/29 00:00 [accepted]
PHST- 2008/02/19 09:00 [pubmed]
PHST- 2008/06/27 09:00 [medline]
PHST- 2008/02/19 09:00 [entrez]
AID - S0953-7562(07)00212-2 [pii]
AID - 10.1016/j.mycres.2007.08.023 [doi]
PST - ppublish
SO  - Mycol Res. 2008 Feb;112(Pt 2):289-96. doi: 10.1016/j.mycres.2007.08.023. Epub 
      2007 Sep 29.