PMID- 18280135
OWN - NLM
STAT- MEDLINE
DCOM- 20081006
LR  - 20080811
IS  - 0955-2863 (Print)
IS  - 0955-2863 (Linking)
VI  - 19
IP  - 9
DP  - 2008 Sep
TI  - Effects of glycinin on IgE-mediated increase of mast cell numbers and histamine 
      release in the small intestine.
PG  - 627-33
LID - 10.1016/j.jnutbio.2007.08.007 [doi]
AB  - Soybean allergy represents a significant health threat to individuals with food 
      allergies. Glycinin, the main storage protein in soybean, has been identified as 
      a major food allergen. The present study was conducted to investigate the 
      mechanism of glycinin-induced hypersensitivity in a swine model. The relationship 
      between glycinin dose and the severity of hypersensitive reactions was also 
      explored. Twenty-four piglets weaned at 18 days were gastric sensitized and 
      subjected to repeated oral challenges with diets containing 0%, 2%, 4% and 8% 
      glycinin. The results showed that dietary supplementation of glycinin reduced 
      piglet performance (P<.01) while increasing occurrence of diarrhea (P<.05) and 
      erythema area (P=.01) in response to an intradermal injection of glycinin. 
      Intestinal mast cell numbers (P<.05) and immunoglobulin E (IgE) levels (P<.05) 
      were increased linearly, whereas the histamine content in intestinal specimens 
      (except in the duodenum) was decreased (P<.01), indicating that more histamine 
      had been released in glycinin-fed piglets than in control. Serum concentrations 
      of total IgE, glycinin-specific IgG1 and interleukin (IL)-4 and IL-10 were also 
      greater (P<.05) in the pigs treated with glycinin. In this study, we found that 
      glycinin-induced hypersensitivity is a predominantly Th2-type immune response, 
      mediated by IgE and associated with increases in intestinal mast cell numbers and 
      histamine release as well as IL-4 and IL-10 concentrations in the serum of 
      sensitized piglets, resulting in diarrhea and reduced performance. The severity 
      of the hypersensitive reactions depends on the dose of glycinin. Higher dose may 
      cause more severe anaphylactic symptoms.
FAU - Sun, Peng
AU  - Sun P
AD  - National Key Laboratory of Animal Nutrition, China Agricultural University, 
      Beijing, 100094, P.R. China.
FAU - Li, Defa
AU  - Li D
FAU - Li, Zheji
AU  - Li Z
FAU - Dong, Bing
AU  - Dong B
FAU - Wang, Fenglai
AU  - Wang F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080214
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Globulins)
RN  - 0 (Soybean Proteins)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9007-93-6 (glycinin)
SB  - IM
MH  - Animals
MH  - Food Hypersensitivity/*immunology
MH  - Globulins/*immunology
MH  - Histamine Release/*drug effects
MH  - Immunoglobulin E/*physiology
MH  - Interleukin-10/blood
MH  - Interleukin-4/blood
MH  - Intestinal Mucosa/cytology
MH  - Intestine, Small/cytology/*drug effects/*immunology
MH  - Male
MH  - Mast Cells/*drug effects
MH  - Soybean Proteins/*immunology
MH  - Sus scrofa
EDAT- 2008/02/19 09:00
MHDA- 2008/10/07 09:00
CRDT- 2008/02/19 09:00
PHST- 2007/05/15 00:00 [received]
PHST- 2007/08/08 00:00 [revised]
PHST- 2007/08/27 00:00 [accepted]
PHST- 2008/02/19 09:00 [pubmed]
PHST- 2008/10/07 09:00 [medline]
PHST- 2008/02/19 09:00 [entrez]
AID - S0955-2863(07)00194-5 [pii]
AID - 10.1016/j.jnutbio.2007.08.007 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2008 Sep;19(9):627-33. doi: 10.1016/j.jnutbio.2007.08.007. Epub 
      2008 Feb 14.