PMID- 18282241
OWN - NLM
STAT- MEDLINE
DCOM- 20080606
LR  - 20211020
IS  - 0041-1132 (Print)
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Linking)
VI  - 48
IP  - 4
DP  - 2008 Apr
TI  - Evaluation of gene expression profiles of immature dendritic cells prepared from 
      peripheral blood mononuclear cells.
PG  - 647-57
LID - 10.1111/j.1537-2995.2007.01615.x [doi]
AB  - BACKGROUND: Dendritic cells (DCs) generated ex vivo from peripheral blood 
      monocytes or mobilized CD34+ cells and intended for clinical immunotherapy are 
      typically characterized by morphologic, phenotypic, and functional assays. Assay 
      results are highly dependent on conditions used to prepare the cells, so there is 
      no standard assay battery for clinical DC products. This study evaluated gene 
      expression profiling for characterization of immature DCs prepared from monocytes 
      that had been elutriated from normal donor peripheral blood mononuclear cells 
      (PBMNCs) immediately after collection or after storage at 4 degrees C for 48 
      hours. STUDY DESIGN AND METHODS: RNA was isolated from fresh and 48-hour-stored 
      PBMNCs, elutriated monocytes, elutriated lymphocytes, and immature DCs from five 
      healthy subjects and was analyzed with a cDNA gene expression microarray with 
      17,500 genes. RESULTS: Unsupervised hierarchical clustering separated the 40 
      products into four groups: one with all 10 immature DCs, one with all 10 
      elutriated lymphocytes, one with 7 PBMNCs, and one with 10 elutriated monocytes 
      and 3 PBMNCs. Within each of the four groups, however, fresh and stored products, 
      or products derived from fresh or stored products, clustered together. Comparison 
      of genes differentially expressed by fresh versus stored products (paired t 
      tests, p < 0.005) found 273 genes that differed between fresh and stored PBMCs, 
      429 between lymphocytes elutriated from fresh versus stored PBMNCs, 711 between 
      monocytes elutriated from fresh versus stored PBMNCs, and 3 between immature DCs 
      prepared from monocytes elutriated from fresh versus stored PBMCs. CONCLUSIONS: 
      This study demonstrates the potential utility of gene expression profiling for 
      characterization of cell therapy products.
FAU - Shin, Jeong Won
AU  - Shin JW
AD  - Department of Transfusion Medicine, NIH Clinical Center, National Institutes of 
      Health, Bethesda, Maryland 20892-1184, USA.
FAU - Jin, Ping
AU  - Jin P
FAU - Fan, Yong
AU  - Fan Y
FAU - Slezak, Stefanie
AU  - Slezak S
FAU - David-Ocampo, Virginia
AU  - David-Ocampo V
FAU - Khuu, Hanh M
AU  - Khuu HM
FAU - Read, Elizabeth J
AU  - Read EJ
FAU - Wang, Ena
AU  - Wang E
FAU - Marincola, Francesco M
AU  - Marincola FM
FAU - Stroncek, David F
AU  - Stroncek DF
LA  - eng
GR  - Z01 CL002095-11/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
DEP - 20080212
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cluster Analysis
MH  - Dendritic Cells/cytology/*metabolism
MH  - Female
MH  - Gene Expression Profiling/*methods
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*cytology
MH  - Oligonucleotide Array Sequence Analysis
PMC - PMC2710576
MID - NIHMS102534
COIS- Conflict-of-interest statement: the authors declare no competing financial 
      interests.
EDAT- 2008/02/20 09:00
MHDA- 2008/06/07 09:00
PMCR- 2009/07/15
CRDT- 2008/02/20 09:00
PHST- 2008/02/20 09:00 [pubmed]
PHST- 2008/06/07 09:00 [medline]
PHST- 2008/02/20 09:00 [entrez]
PHST- 2009/07/15 00:00 [pmc-release]
AID - TRF01615 [pii]
AID - 10.1111/j.1537-2995.2007.01615.x [doi]
PST - ppublish
SO  - Transfusion. 2008 Apr;48(4):647-57. doi: 10.1111/j.1537-2995.2007.01615.x. Epub 
      2008 Feb 12.