PMID- 18283042
OWN - NLM
STAT- MEDLINE
DCOM- 20080428
LR  - 20080402
IS  - 1460-2180 (Electronic)
IS  - 0143-3334 (Linking)
VI  - 29
IP  - 4
DP  - 2008 Apr
TI  - Detection of chromosome changes in pathology archives: an application of 
      microwave-assisted fluorescence in situ hybridization to human carcinogenesis 
      studies.
PG  - 681-7
LID - 10.1093/carcin/bgn046 [doi]
AB  - Pathology archives provide unique and abundant opportunities to investigate human 
      carcinogenesis and identify potential targets for cancer therapy. Microwaving was 
      introduced into various procedures used in histopathology two decades ago, 
      although the precise mechanisms underlying its effectiveness in any of the 
      procedures, including antigen retrieval, acceleration of fixation and nucleic 
      acid hybridization, are not known. Since microwaving was first applied to 
      fluorescence in situ hybridization (FISH), many pathologists and researchers have 
      enjoyed the benefits of excellent preservation of histological structures as well 
      as good retrieval of FISH signals by this method. Microwave-assisted fluorescence 
      in situ hybridization (MW-FISH) has proved to be especially useful in 
      retrospective investigations of tissues fixed and preserved for long periods of 
      time, and the success rates in the randomly selected pathology archives have been 
      greater (70-95%) than by the conventional protocol (</=40%) The MW-FISH protocol 
      and current availability of human genome information together with information on 
      a variety of other histopathological attributes have paved the way to exploration 
      of specific, large-scale genomic changes in human tumor tissue, even in the 
      incipient stage. In practice, this protocol is very useful for retrospective 
      surveillance of amplicons in tumor tissue by using hundreds of bacterial 
      artificial chromosome clones and many specimens in the form of a tissue 
      microarray. Effective retrieval of specific genome-wide amplicon profiles from 
      human tumors stored unaware in ordinary pathology laboratories would help to 
      further stratify tumors so that individually tailored treatment strategies would 
      become feasible in clinical settings.
FAU - Sugimura, Haruhiko
AU  - Sugimura H
AD  - Department of Pathology, Hamamatsu University School of Medicine, 1-20-1 
      Handayama, Hamamatsu, Shizuoka 431-3192, Japan. hsugimur@hama-med.ac.jp
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20080218
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
SB  - IM
MH  - Chromosomes, Human/*genetics/radiation effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Microwaves
MH  - Neoplasms/*genetics/*pathology
MH  - Polymorphism, Single Nucleotide
RF  - 57
EDAT- 2008/02/20 09:00
MHDA- 2008/04/29 09:00
CRDT- 2008/02/20 09:00
PHST- 2008/02/20 09:00 [pubmed]
PHST- 2008/04/29 09:00 [medline]
PHST- 2008/02/20 09:00 [entrez]
AID - bgn046 [pii]
AID - 10.1093/carcin/bgn046 [doi]
PST - ppublish
SO  - Carcinogenesis. 2008 Apr;29(4):681-7. doi: 10.1093/carcin/bgn046. Epub 2008 Feb 
      18.