PMID- 18283540 OWN - NLM STAT- MEDLINE DCOM- 20081118 LR - 20211020 IS - 0016-6707 (Print) IS - 0016-6707 (Linking) VI - 134 IP - 3 DP - 2008 Nov TI - Comparative gene mapping in cattle, Indian muntjac, and Chinese muntjac by fluorescence in situ hybridization. PG - 345-51 LID - 10.1007/s10709-008-9242-1 [doi] AB - The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n = 6 in the female and 2n = 7 in the male. The karyotypic evolution of Indian muntjac via extensive tandem fusions and several centric fusions are well documented by molecular cytogenetic studies mainly utilizing chromosome paints. To achieve higher resolution mapping, a set of 42 different genomic clones coding for 37 genes and the nucleolar organizer region were used to examine homologies between the cattle (2n = 60), human (2n = 46), Indian muntjac (2n = 6/7) and Chinese muntjac (2n = 46) karyotypes. These genomic clones were mapped by fluorescence in situ hybridization (FISH). Localization of genes on all three pairs of M. m. vaginalis chromosomes and on the acrocentric chromosomes of M. reevesi allowed not only the analysis of the evolution of syntenic regions within the muntjac genus but also allowed a broader comparison of synteny with more distantly related species, such as cattle and human, to shed more light onto the evolving genome organization. FAU - Murmann, Andrea E AU - Murmann AE AD - Department of Medicine, Section Hematology/Oncology, University of Chicago, Chicago, IL 60637, USA. amurmann@uchicago.edu FAU - Mincheva, Antoaneta AU - Mincheva A FAU - Scheuermann, Markus O AU - Scheuermann MO FAU - Gautier, Mathieu AU - Gautier M FAU - Yang, Fentang AU - Yang F FAU - Buitkamp, Johannes AU - Buitkamp J FAU - Strissel, Pamela L AU - Strissel PL FAU - Strick, Reiner AU - Strick R FAU - Rowley, Janet D AU - Rowley JD FAU - Lichter, Peter AU - Lichter P LA - eng PT - Comparative Study PT - Journal Article DEP - 20080219 PL - Netherlands TA - Genetica JT - Genetica JID - 0370740 SB - IM MH - Animals MH - Cattle MH - Chromosome Mapping MH - Chromosomes MH - Chromosomes, Artificial, Bacterial MH - Evolution, Molecular MH - Female MH - Humans MH - In Situ Hybridization, Fluorescence MH - Karyotyping MH - Male MH - Muntjacs/classification/*genetics EDAT- 2008/02/20 09:00 MHDA- 2008/11/19 09:00 CRDT- 2008/02/20 09:00 PHST- 2007/09/30 00:00 [received] PHST- 2008/01/26 00:00 [accepted] PHST- 2008/02/20 09:00 [pubmed] PHST- 2008/11/19 09:00 [medline] PHST- 2008/02/20 09:00 [entrez] AID - 10.1007/s10709-008-9242-1 [doi] PST - ppublish SO - Genetica. 2008 Nov;134(3):345-51. doi: 10.1007/s10709-008-9242-1. Epub 2008 Feb 19.