PMID- 18284633
OWN - NLM
STAT- MEDLINE
DCOM- 20090723
LR  - 20080821
IS  - 1365-2265 (Electronic)
IS  - 0300-0664 (Linking)
VI  - 69
IP  - 2
DP  - 2008 Aug
TI  - Role of macrophage infiltration in the orbital fat of patients with Graves' 
      ophthalmopathy.
PG  - 332-7
LID - 10.1111/j.1365-2265.2008.03219.x [doi]
AB  - OBJECTIVE: Infiltration of the retro-ocular space by inflammatory cells, 
      accumulation of glycosaminoglycans, and the overabundance of orbital adipose 
      tissue are characteristic findings in Graves' ophthalmopathy (GO). The cause of 
      macrophage infiltration in the orbital adipose tissue of patients with GO remains 
      to be elucidated. DESIGN: Immunohistochemistry of orbital adipose tissues with 
      anti-CD68 was used for determining macrophage infiltration pattern and cell 
      counts. Quantitative real-time PCR was used for analysing mRNA expression. 
      Correlation of macrophage infiltration with the duration of GO and mRNA 
      expression were also determined. PATIENTS: Fifteen subjects with GO who underwent 
      orbital decompression were recruited. Six patients without thyroid history who 
      underwent elective orbital surgery were enrolled as controls. MEASUREMENTS: 
      Histological distribution of macrophages, macrophage cell counts, CD68 and 
      monocyte chemoattractant protein-1 (MCP-1) mRNA levels, and duration of GO. 
      RESULTS: We demonstrated that macrophage infiltration in orbital fat from 
      patients with GO was higher than controls (P = 0.005). The infiltration of 
      macrophages was located primarily around blood vessels and between mature 
      adipocytes. Macrophage infiltration did not attenuate in GO of long duration. We 
      also found that the expression of MCP-1 was higher in GO orbital fat than that in 
      the orbital fat of controls (P = 0.047) and the infiltration of macrophages in 
      adipose tissue from patients with GO was positively correlated with expression of 
      MCP-1 mRNA (r = 0.546, P = 0.035). CONCLUSION: Macrophage infiltration may play 
      an important role in the pathogenesis of GO via over-expression of MCP-1.
FAU - Chen, Mei-Hsiu
AU  - Chen MH
AD  - Graduate Institute of Clinical Medicine, National Taiwan University Medical 
      College, Taipei, Taiwan.
FAU - Chen, Ming-Hong
AU  - Chen MH
FAU - Liao, Shu-Lang
AU  - Liao SL
FAU - Chang, Tien-Chun
AU  - Chang TC
FAU - Chuang, Lee-Ming
AU  - Chuang LM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080212
PL  - England
TA  - Clin Endocrinol (Oxf)
JT  - Clinical endocrinology
JID - 0346653
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CCL2 protein, human)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Adipose Tissue/*immunology/metabolism/pathology
MH  - Adult
MH  - Antigens, CD/genetics/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/genetics/metabolism
MH  - Cell Count
MH  - Cell Movement/genetics/*physiology
MH  - Chemokine CCL2/genetics/metabolism
MH  - Female
MH  - Graves Ophthalmopathy/genetics/immunology/metabolism/*pathology
MH  - Humans
MH  - Macrophages/immunology/metabolism/*pathology
MH  - Male
MH  - Middle Aged
MH  - *Orbit/pathology
MH  - Up-Regulation
MH  - Young Adult
EDAT- 2008/02/21 09:00
MHDA- 2009/07/25 09:00
CRDT- 2008/02/21 09:00
PHST- 2008/02/21 09:00 [pubmed]
PHST- 2009/07/25 09:00 [medline]
PHST- 2008/02/21 09:00 [entrez]
AID - CEN3219 [pii]
AID - 10.1111/j.1365-2265.2008.03219.x [doi]
PST - ppublish
SO  - Clin Endocrinol (Oxf). 2008 Aug;69(2):332-7. doi: 
      10.1111/j.1365-2265.2008.03219.x. Epub 2008 Feb 12.