PMID- 18284841
OWN - NLM
STAT- MEDLINE
DCOM- 20080529
LR  - 20220408
IS  - 1027-3719 (Print)
IS  - 1027-3719 (Linking)
VI  - 12
IP  - 3
DP  - 2008 Mar
TI  - Microscopic-observation drug susceptibility assay provides rapid and reliable 
      identification of MDR-TB.
PG  - 332-7
AB  - BACKGROUND: Liquid culture systems are more rapid and sensitive for both the 
      detection and drug susceptibility testing (DST) of Mycobacterium tuberculosis. 
      SETTING: St Peter's TB Specialised Hospital and public health laboratory, Addis 
      Ababa. OBJECTIVE: To compare the microscopic-observation drug susceptibility 
      (MODS) assay with the BACTEC-MGIT 960 system for isoniazid and rifampicin DST 
      (i.e., multidrug-resistant tuberculosis [MDR-TB] identification) of M. 
      tuberculosis. DESIGN: The evaluation was based on 58 smear- and culture-positive 
      sputum samples from patients diagnosed in Addis Ababa, Ethiopia. BACTEC-MGIT was 
      used as the reference standard. RESULTS: For the detection of MDR-TB, MODS has a 
      sensitivity, specificity and accuracy rate of respectively 95%, 100% and 98.3% 
      (kappa 0.981, concordance 98.3%). Concurrent culture detection and DST results 
      are obtained in a median of 9 days with MODS, while indirect DST results with 
      BACTEC-MGIT are obtained in a median of 8 days (this does not include time to 
      primary isolate). CONCLUSION: MODS is an accurate, rapid and relatively 
      inexpensive method for the identification of MDR-TB.
FAU - Ejigu, G S
AU  - Ejigu GS
AD  - Medical Faculty, College of Health Sciences, Hawassa University, Awassa, 
      Ethiopia. girumdvm@yahoo.com
FAU - Woldeamanuel, Y
AU  - Woldeamanuel Y
FAU - Shah, N S
AU  - Shah NS
FAU - Gebyehu, M
AU  - Gebyehu M
FAU - Selassie, A
AU  - Selassie A
FAU - Lemma, E
AU  - Lemma E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - France
TA  - Int J Tuberc Lung Dis
JT  - The international journal of tuberculosis and lung disease : the official journal 
      of the International Union against Tuberculosis and Lung Disease
JID - 9706389
RN  - 0 (Antitubercular Agents)
RN  - V83O1VOZ8L (Isoniazid)
RN  - VJT6J7R4TR (Rifampin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antitubercular Agents/*pharmacology
MH  - Child
MH  - Drug Resistance, Multiple, Bacterial
MH  - Female
MH  - Humans
MH  - Isoniazid/*pharmacology
MH  - Male
MH  - Microbial Sensitivity Tests/*methods
MH  - Middle Aged
MH  - Mycobacterium tuberculosis/*drug effects
MH  - Rifampin/*pharmacology
MH  - Sensitivity and Specificity
MH  - Sputum/microbiology
MH  - Tuberculosis, Multidrug-Resistant/*diagnosis
EDAT- 2008/02/21 09:00
MHDA- 2008/05/30 09:00
CRDT- 2008/02/21 09:00
PHST- 2008/02/21 09:00 [pubmed]
PHST- 2008/05/30 09:00 [medline]
PHST- 2008/02/21 09:00 [entrez]
PST - ppublish
SO  - Int J Tuberc Lung Dis. 2008 Mar;12(3):332-7.