PMID- 18285259
OWN - NLM
STAT- MEDLINE
DCOM- 20080408
LR  - 20091103
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 129
IP  - 3
DP  - 2008 Mar
TI  - Analysis of chromosome 1p abnormalities in renal oncocytomas by loss of 
      heterozygosity studies: correlation with conventional cytogenetics and 
      fluorescence in situ hybridization.
PG  - 377-82
LID - 10.1309/KC2465ANDWVAXYDM [doi]
AB  - We previously showed by cytogenetics and fluorescence in situ hybridization 
      (FISH) that the most common chromosomal abnormality in renal oncocytomas is loss 
      of chromosome 1 or 1p. In the present study, we evaluated chromosome 1 by loss of 
      heterozygosity (LOH) studies. DNA was extracted from paraffin sections. Three 
      microsatellite markers were used: D1S508, D1S199, and D1S2734. The regions 
      targeted by FISH probes and LOH markers were close to each other but not 
      overlapping. Among 16 tumors evaluated by all 3 techniques, in 2 cases, LOH could 
      not be interpreted. LOH was detected in at least 1 locus in 12 (86%) of 14 renal 
      oncocytomas studied, with other loci being noninformative or not interpretable (1 
      case). In 2 cases, the LOH results were inconclusive. These results provide 
      further evidence to support widespread abnormalities in chromosome 1p in renal 
      oncocytoma. Determining whether such abnormalities are unique to renal 
      oncocytomas or are also present in other tumors requires further studies.
FAU - Picken, Maria M
AU  - Picken MM
AD  - Department of Pathology, Loyola University Medical Center, Maywood, IL 60153, 
      USA. mpicken@lumc.edu
FAU - Chyna, Brent
AU  - Chyna B
FAU - Flanigan, Robert C
AU  - Flanigan RC
FAU - Lee, John M
AU  - Lee JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adenoma, Oxyphilic/*genetics
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kidney Neoplasms/*genetics
MH  - Loss of Heterozygosity
MH  - Male
MH  - Microsatellite Repeats
MH  - Middle Aged
MH  - Polymerase Chain Reaction
EDAT- 2008/02/21 09:00
MHDA- 2008/04/09 09:00
CRDT- 2008/02/21 09:00
PHST- 2008/02/21 09:00 [pubmed]
PHST- 2008/04/09 09:00 [medline]
PHST- 2008/02/21 09:00 [entrez]
AID - U350641534756KG8 [pii]
AID - 10.1309/KC2465ANDWVAXYDM [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2008 Mar;129(3):377-82. doi: 10.1309/KC2465ANDWVAXYDM.