PMID- 18287408 OWN - NLM STAT- MEDLINE DCOM- 20080602 LR - 20211020 IS - 0016-6731 (Print) IS - 0016-6731 (Linking) VI - 178 IP - 2 DP - 2008 Feb TI - Completely distinguishing individual A-genome chromosomes and their karyotyping analysis by multiple bacterial artificial chromosome - fluorescence in situ hybridization. PG - 1117-22 LID - 10.1534/genetics.107.083576 [doi] AB - Twenty bacterial artificial chromosome (BAC) clones that could produce bright signals and no or very low fluorescence in situ hybridization (FISH) background were identified from Gossypium arboreum cv. JLZM, and G. hirsutum accession (acc.) TM-1 and 0-613-2R. Combining with 45S and 5S rDNA, a 22-probe cocktail that could identify all 13 G. arboreum chromosomes simultaneously was developed. According to their homology with tetraploid cotton, the G. arboreum chromosomes were designated as A1-A13, and a standard karyotype analysis of G. arboreum was presented. These results demonstrated an application for multiple BAC-FISH in cotton cytogenetic studies and a technique to overcome the problem of simultaneous chromosome recognition in mitotic cotton cells. FAU - Wang, Kai AU - Wang K AD - National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China. FAU - Guan, Bing AU - Guan B FAU - Guo, Wangzhen AU - Guo W FAU - Zhou, Baoliang AU - Zhou B FAU - Hu, Yan AU - Hu Y FAU - Zhu, Yichao AU - Zhu Y FAU - Zhang, Tianzhen AU - Zhang T LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Genetics JT - Genetics JID - 0374636 RN - 0 (DNA, Plant) RN - 0 (DNA, Ribosomal) SB - IM MH - *Chromosomes, Artificial, Bacterial MH - Chromosomes, Plant/genetics MH - Cloning, Molecular MH - DNA, Plant/genetics MH - DNA, Ribosomal/genetics MH - Gossypium/*genetics MH - In Situ Hybridization, Fluorescence MH - Karyotyping MH - Nucleic Acid Hybridization PMC - PMC2248359 EDAT- 2008/02/22 09:00 MHDA- 2008/06/03 09:00 PMCR- 2008/05/01 CRDT- 2008/02/22 09:00 PHST- 2008/02/22 09:00 [pubmed] PHST- 2008/06/03 09:00 [medline] PHST- 2008/02/22 09:00 [entrez] PHST- 2008/05/01 00:00 [pmc-release] AID - 178/2/1117 [pii] AID - gen17821117 [pii] AID - 10.1534/genetics.107.083576 [doi] PST - ppublish SO - Genetics. 2008 Feb;178(2):1117-22. doi: 10.1534/genetics.107.083576.