PMID- 18288283
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1687-4757 (Print)
IS  - 1687-4765 (Electronic)
VI  - 2008
DP  - 2008
TI  - Cardiac PPARalpha Protein Expression is Constant as Alternate Nuclear Receptors 
      and PGC-1 Coordinately Increase During the Postnatal Metabolic Transition.
PG  - 279531
LID - 10.1155/2008/279531 [doi]
LID - 279531
AB  - Gene expression data obtained in mouse heart indicate that increased expression 
      for the nuclear receptor, peroxisomal proliferator activated receptor alpha 
      (PPARalpha), prompts the postnatal transition from predominantly carbohydrate to 
      fatty acid oxidation preference. However, no phenotypic or proteomic data are 
      available to confirm downstream signaling and metabolic transition in mice. We 
      studied the hypothesis that shifts in nuclear receptor expression trigger the 
      newborn metabolic switch in a newborn sheep. This species is well characterized 
      with regards to developmental changes in substrate oxidative metabolism. Heart 
      tissues from fetal (130 days gestation), newborn </=24 hours, and 30-day old 
      lambs were evaluated for protein expression from multiple enzymes controlling 
      oxidative metabolism as well as principal nuclear receptors and coactivators. 
      Although muscle and liver type carnitine palmitoyl transferases I showed no 
      significant changes to correspond to the metabolic transition, hexokinase II 
      protein content showed a profound transient drop, and pyruvate dehydrogenase 
      kinase steadily increased. PPARalpha showed no increases preceding or during the 
      transition, while peroxisomal proliferator activated receptor gamma coactivator-1 
      (PGC-1) increased approximately 20-fold transiently in newborn heart in 
      conjunction with significant increases in thyroid hormone receptor alpha1 and 
      retinoid-activated receptor alpha. These data challenge the paradigm that 
      increases in PPARalpha prompt the postnatal metabolic switch, and suggest that 
      other nuclear receptors play a major role. As thyroid hormone (TH) modulates 
      PGC-1 expression in sheep during development, these data further suggest that 
      well-characterized perinatal TH surge in sheep contributes to this metabolic 
      switch.
FAU - Buroker, Norman E
AU  - Buroker NE
AD  - Department of Cardiology, Children's Hospital and Regional Medical Center, 4800 
      Sand Point Way N.E., Seattle, WA 98105, USA.
FAU - Ning, Xue-Han
AU  - Ning XH
FAU - Portman, Michael
AU  - Portman M
LA  - eng
GR  - R01 HL060666/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - PPAR Res
JT  - PPAR research
JID - 101269101
PMC - PMC2233871
EDAT- 2008/02/22 09:00
MHDA- 2008/02/22 09:01
PMCR- 2008/01/17
CRDT- 2008/02/22 09:00
PHST- 2007/06/20 00:00 [received]
PHST- 2007/07/22 00:00 [accepted]
PHST- 2008/02/22 09:00 [pubmed]
PHST- 2008/02/22 09:01 [medline]
PHST- 2008/02/22 09:00 [entrez]
PHST- 2008/01/17 00:00 [pmc-release]
AID - 10.1155/2008/279531 [doi]
PST - ppublish
SO  - PPAR Res. 2008;2008:279531. doi: 10.1155/2008/279531.