PMID- 18288357
OWN - NLM
STAT- MEDLINE
DCOM- 20150516
LR  - 20181201
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 60
IP  - 1
DP  - 2008 Feb 25
TI  - [Expressions of atherosclerosis-related genes in aorta in young apoE/LDLR double 
      knockout mice].
PG  - 43-50
AB  - To systematically clarify the effects of apolipoprotein E (aopE) and low-density 
      lipoprotein receptor (LDLR) gene mutant on hyperlipidemia, vascular inflammation 
      impairment and pathogenesis of atherosclerosis (AS), total RNA was isolated from 
      fresh aortas of young apoE/LDLR double knockout (apoE(-/-)/LDLR(-/-)) and wild 
      type (WT) mice using TRIzol reagent. Then RNA was reversely transcribed to 
      first-strand cDNA by reverse transcriptase for reverse transcription polymerase 
      chain reaction (RT-PCR) and real-time RT-PCR. Primer pairs were designed using 
      primer design software according to the gene sequences available in GenBank. 
      beta-actin was used as an internal control. Then RT-PCR assay was used to analyze 
      the expression patterns of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha 
      (TNF-alpha), nuclear factor-kappaB (NF-kappaB), granulocyte-macrophage colony-stimulating 
      factor (GM-CSF), CD36, endothelin-1 (ET-1), toll-like receptor 2 (TLR2), monocyte 
      chemoattractant protein-1 (MCP-1), vascular adhesion molecule-1 (VCAM-1), 
      intercellular adhesion molecule-1 (ICAM-1) and platelet-derived growth factor-alpha 
      (PDGF-alpha). SYBR Green quantitative real-time RT-PCR was used to validate gene 
      expressions identified by RT-PCR. Blood samples were taken from the retro-orbital 
      venous plexus, and serum levels of total cholesterol (TC), triglyceride (TG), 
      low-density lipoprotein (LDL) and high-density lipoprotein (HDL) were measured by 
      using biochemical techniques. Serum concentrations of circulating TNF-alpha, IL-1beta 
      and oxidized LDL (ox-LDL) were determined by ELISA. Frozen sections of aortic 
      sinus were stained with Sudan IV to visualize intimal fatty lesions. The results 
      showed that the relative expressions of IL-1beta, GM-CSF, ET-1, TLR2, CD36, MCP-1, 
      ICAM-1 and VCAM-1 in apoE(-/-)/LDLR(-/-) mice at the age of 1 month were higher 
      than those in age-matched WT mice (P<0.05, P<0.01), respectively. The expressions 
      of PDGF-alpha and TNF-alpha in apoE(-/-)/LDLR(-/-) mice at the age of 2 months were 
      up-regulated compared to those in age-matched WT mice (P<0.05). All the 
      expressions of target genes continued to be up-regulated (P<0.05, P<0.01) except 
      that ET-1 expression at the age of 2 months, TLR2, VCAM-1 and ICAM-1 expressions 
      at the age of 3 months were down-regulated to that in WT mice. NF-kappaB expression 
      had no significant changes between two genotype mice at different ages. All the 
      gene expressions kept unchanged in WT mice at different ages, except that IL-1b 
      expressions were slightly up-regulated at the ages of 2 and 3 months. Serum 
      levels of TC, TG, LDL, HDL, TNF-alpha, IL-1beta and ox-LDL in apoE(-/-)/LDLR(-/-) mice 
      at different ages were higher than those in age-matched WT mice (P<0.05, P<0.01), 
      and were increasing with age. Primary atherosclerotic lesions were observed in 
      1-month old apoE(-/-)/LDLR(-/-) mice and were progressing with age. There were no 
      lesions observed in all WT mice at different ages. The data suggest that 
      hyperlipidemia due to apoE and LDLR gene mutant may stimulate the temporal 
      expressions of AS-related genes and contribute to primary atherogenetic lesions 
      and vascular inflammation impairment.
FAU - Dai, Xue-Dong
AU  - Dai XD
AD  - The Key Laboratory of Animal Resistant Biology of Shandong Province, College of 
      Life Sciences, Shandong Normal University, Jinan 250014, China.
FAU - Yin, Miao
AU  - Yin M
FAU - Jing, Wen
AU  - Jing W
FAU - DU, Hui-Qin
AU  - DU HQ
FAU - Ye, Hong-Yan
AU  - Ye HY
FAU - Shang, Yun-Ju
AU  - Shang YJ
FAU - Zhang, Liang
AU  - Zhang L
FAU - Zou, Yan-Yan
AU  - Zou YY
FAU - Qu, Zhi-Ping
AU  - Qu ZP
FAU - Pan, Jie
AU  - Pan J
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (Apolipoproteins E)
RN  - 0 (CD36 Antigens)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Endothelin-1)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (NF-kappa B)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Receptors, LDL)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 0 (apolipoprotein E1)
RN  - 0 (oxidized low density lipoprotein)
RN  - 0 (platelet-derived growth factor A)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Aorta/*metabolism
MH  - Apolipoproteins E/genetics
MH  - Atherosclerosis/*genetics
MH  - CD36 Antigens/metabolism
MH  - Chemokine CCL2/metabolism
MH  - Endothelin-1/metabolism
MH  - Gene Expression
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Hyperlipidemias/*metabolism
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Interleukin-1beta/blood/metabolism
MH  - Lipoproteins, LDL/blood
MH  - Mice
MH  - Mice, Knockout
MH  - NF-kappa B/metabolism
MH  - Platelet-Derived Growth Factor
MH  - Receptors, LDL/genetics
MH  - Toll-Like Receptor 2/metabolism
MH  - Tumor Necrosis Factor-alpha/blood/metabolism
MH  - Vascular Cell Adhesion Molecule-1/metabolism
EDAT- 2008/02/22 09:00
MHDA- 2015/05/20 06:00
CRDT- 2008/02/22 09:00
PHST- 2008/02/22 09:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
PHST- 2008/02/22 09:00 [entrez]
PST - ppublish
SO  - Sheng Li Xue Bao. 2008 Feb 25;60(1):43-50.