PMID- 18289664
OWN - NLM
STAT- MEDLINE
DCOM- 20080618
LR  - 20181201
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 29
IP  - 14
DP  - 2008 May
TI  - The influence of proepicardial cells on the osteogenic potential of marrow 
      stromal cells in a three-dimensional tubular scaffold.
PG  - 2203-16
LID - 10.1016/j.biomaterials.2008.01.025 [doi]
AB  - It is well established that the process of neovascularization or neoangiogenesis 
      is coupled to the development and maturation of bone. Bone marrow stromal cells 
      (BMSCs) or mesenchymal stem cells (MSCs) comprise a heterogeneous population of 
      cells that can be differentiated in vitro into both mesenchymal and 
      non-mesenchymal cell lineages. When both rat BMSCs and quail proepicardia (PEs) 
      were seeded onto a three-dimensional (3-D) tubular scaffold engineered from 
      aligned collagen type I strands and co-cultured in osteogenic media, the 
      maturation and co-differentiation into osteoblastic and vascular cell lineages 
      were observed. In addition, these cells produced abundant mineralized 
      extracellular matrix materials and vessel-like structures. BMSCs were seeded at a 
      density of 2 x 10(6)cells/15 mm tube and cultured in basal media for 3 days. 
      Subsequently, on day 3, PEs were seeded onto the same tubes and the co-culture 
      was continued for another 3, 6 or 9 days either in basal or in osteogenic media. 
      Differentiated cells were subjected to immunohistochemical, cytochemical and 
      biochemical analyses. Phenotypic induction was analyzed at mRNA level by reverse 
      transcriptase quantitative polymerase chain reaction (RT-qPCR). 
      Immunolocalization of key osteogenic and vasculogenic lineage specific markers 
      were examined using confocal scanning laser microscopy. In osteogenic tube 
      cultures, both early and late osteogenic markers were observed and were 
      reminiscent of in vivo expression pattern. Alkaline phosphatase activity and 
      calcium content significantly increased over the observed period of time in 
      osteogenic medium. Abundant interlacing fascicles of QCPN, QH1, isolectin and 
      alpha-smooth muscle actin (alpha-SMA) positive cells were observed in these tube 
      cultures. These cells formed extensive arborizations of nascent capillary-like 
      structures and were seen amidst the developing osteoblasts in osteogenic 
      cultures. The 3-D culture system not only generated de novo vessel-like 
      structures but also augmented the maturation and differentiation of BMSCs into 
      osteoblasts. Thus, this novel co-culture system provides a useful in vitro model 
      to investigate the functional role and effects of neovascularization in the 
      proliferation, differentiation and maturation of BMSC derived osteoblasts.
FAU - Valarmathi, Mani T
AU  - Valarmathi MT
AD  - Department of Cell and Developmental Biology & Anatomy, School of Medicine, 
      University of South Carolina, Columbia, South Carolina, USA. 
      valarmathi64@hotmail.com
FAU - Yost, Michael J
AU  - Yost MJ
FAU - Goodwin, Richard L
AU  - Goodwin RL
FAU - Potts, Jay D
AU  - Potts JD
LA  - eng
PT  - Journal Article
DEP - 20080304
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Collagen Type I)
RN  - 0 (RNA, Messenger)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alkaline Phosphatase/analysis/metabolism
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Calcium/analysis/metabolism
MH  - Cell Culture Techniques
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Collagen Type I/chemistry
MH  - Immunohistochemistry
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/metabolism/physiology
MH  - Osteogenesis/*physiology
MH  - Pericardium/*cytology
MH  - Quail
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Stromal Cells/*cytology/metabolism
MH  - Time Factors
MH  - Tissue Engineering/*methods
EDAT- 2008/02/22 09:00
MHDA- 2008/06/19 09:00
CRDT- 2008/02/22 09:00
PHST- 2007/11/12 00:00 [received]
PHST- 2008/01/27 00:00 [accepted]
PHST- 2008/02/22 09:00 [pubmed]
PHST- 2008/06/19 09:00 [medline]
PHST- 2008/02/22 09:00 [entrez]
AID - S0142-9612(08)00055-0 [pii]
AID - 10.1016/j.biomaterials.2008.01.025 [doi]
PST - ppublish
SO  - Biomaterials. 2008 May;29(14):2203-16. doi: 10.1016/j.biomaterials.2008.01.025. 
      Epub 2008 Mar 4.