PMID- 18290330
OWN - NLM
STAT- MEDLINE
DCOM- 20080404
LR  - 20181201
IS  - 0065-2598 (Print)
IS  - 0065-2598 (Linking)
VI  - 614
DP  - 2008
TI  - Formation of capillary tube-like structures on micropatterned biomaterials.
PG  - 199-205
LID - 10.1007/978-0-387-74911-2_23 [doi]
AB  - The survival of three-dimensional tissue requires a vascular network to provide 
      transport of oxygen and metabolic byproduct. Here, we report a new approach to 
      create capillary blood vessels in vitro on biomaterials suitable for use as 
      scaffolds in engineering tissues. Endothelial cells were cultured on chemical and 
      topographical patterns of micro-sized grooves on gelatin. Selective attachment 
      and spreading of cells within the grooves was ensured by microcontact printing 
      the plateau regions with cell resistant PEG/PLA 
      (polyethyleneglycol-L-polylacticacid). Human microvascular endothelial cells 
      plated on these patterned biomaterials attached and spread exclusively within the 
      grooves. These topographical features promote endothelial cells to form capillary 
      tube-like structures. The results demonstrated that capillary structures formed 
      on biomaterials are useful for engineering vascularized tissues.
FAU - Gao, Dahai
AU  - Gao D
AD  - Department of Chemical Engineering, University of Cincinnati, Cincinnati, OH, 
      USA.
FAU - Kumar, Girish
AU  - Kumar G
FAU - Co, Carlos
AU  - Co C
FAU - Ho, Chia-Chi
AU  - Ho CC
LA  - eng
GR  - HL-084648/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Adv Exp Med Biol
JT  - Advances in experimental medicine and biology
JID - 0121103
RN  - 0 (Biocompatible Materials)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 0 (Proteins)
RN  - 136832-63-8 (5-chloromethylfluorescein)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 459TN2L5F5 (poly(lactide))
RN  - 9000-70-8 (Gelatin)
RN  - T3C89M417N (Glutaral)
SB  - IM
MH  - *Biocompatible Materials
MH  - Capillaries/*cytology/*growth & development
MH  - Cells, Cultured
MH  - Cross-Linking Reagents/pharmacology
MH  - Endothelial Cells/cytology
MH  - Endothelium, Vascular/cytology
MH  - Fluoresceins/metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - Fluorescent Dyes/metabolism
MH  - Gelatin/chemistry
MH  - Glutaral/pharmacology
MH  - Humans
MH  - Lactic Acid/chemistry
MH  - Polyesters
MH  - Polyethylene Glycols/chemistry
MH  - Polyglycolic Acid/chemistry
MH  - Polymers/chemistry
MH  - Proteins/metabolism
MH  - Time Factors
EDAT- 2008/02/23 09:00
MHDA- 2008/04/05 09:00
CRDT- 2008/02/23 09:00
PHST- 2008/02/23 09:00 [pubmed]
PHST- 2008/04/05 09:00 [medline]
PHST- 2008/02/23 09:00 [entrez]
AID - 10.1007/978-0-387-74911-2_23 [doi]
PST - ppublish
SO  - Adv Exp Med Biol. 2008;614:199-205. doi: 10.1007/978-0-387-74911-2_23.