PMID- 18293173
OWN - NLM
STAT- MEDLINE
DCOM- 20080331
LR  - 20181201
IS  - 1607-8438 (Electronic)
IS  - 0300-8207 (Linking)
VI  - 49
IP  - 1
DP  - 2008
TI  - Effects of cyclic mechanical stretching on the mRNA expression of 
      tendon/ligament-related and osteoblast-specific genes in human mesenchymal stem 
      cells.
PG  - 7-14
LID - 10.1080/03008200701818561 [doi]
AB  - The purpose of this study was to explore the influences of cyclic mechanical 
      stretching on the mRNA expressions of tendon/ligament-related and 
      osteoblast-specific marker genes in human MSCs seeded onto a collagen type 
      I-coated surface. The stretch-induced mRNA expressions of mesenchymal stem cell 
      protein (MSCP), matrix metalloproteinase-3 (MMP-3), and marker genes related to 
      tendon/ligament cells (type I collagen, type III collagen, and tenascin-C) and 
      those typical of osteoblasts (core binding factor alpha 1 (Cbfa1), alkaline 
      phosphatase (ALP), and osteocalcin (OCN)) were analyzed by quantitative real-time 
      PCR. The results revealed significant downregulation of MSCP and upregulation of 
      MMP-3 genes in MSCs subjected to mechanical loading, regardless of the magnitude 
      of the stretching (high or low). Moreover, the typical marker genes of the 
      osteoblast lineage were upregulated by low-magnitude stretching, whereas 
      tendon/ligament-related genes were upregulated by high-magnitude stretching for a 
      long period. Cbfa1 and ALP were upregulated starting as early at 8 hr, followed 
      by a downward trend and no significant change in expression at the other time 
      points. The mRNA expressions of type I collagen, type III collagen, and 
      tenascin-C significantly increased in MSCs subjected to 10% stretching for 48 hr, 
      and this effect still existed after the stretched cells had rested for 48 hr. 
      This study demonstrated the effect of cyclic mechanical stretching on 
      differential transcription of marker genes related to different cell lineages. 
      Low-magnitude stretching increased mRNA expressions of Cbfa1 and ALP and was 
      possibly involved in the early osteoblastic differentiation of MSCs, whereas 
      high-magnitude stretching upregulated the mRNA expressions of 
      tendon/ligament-related genes.
FAU - Chen, Yi-Jane
AU  - Chen YJ
AD  - School of Dentistry, College of Medicine, National Taiwan University, Taipei, 
      Taiwan.
FAU - Huang, Chien-Hsun
AU  - Huang CH
FAU - Lee, I-Chi
AU  - Lee IC
FAU - Lee, Yu-Tsang
AU  - Lee YT
FAU - Chen, Min-Huey
AU  - Chen MH
FAU - Young, Tai-Horng
AU  - Young TH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Connect Tissue Res
JT  - Connective tissue research
JID - 0365263
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tenascin)
RN  - 104982-03-8 (Osteocalcin)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Collagen/*metabolism
MH  - Core Binding Factor Alpha 1 Subunit/metabolism
MH  - Flow Cytometry
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Osteocalcin/metabolism
MH  - RNA, Messenger/isolation & purification/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Stress, Mechanical
MH  - Tenascin/metabolism
EDAT- 2008/02/23 09:00
MHDA- 2008/04/01 09:00
CRDT- 2008/02/23 09:00
PHST- 2008/02/23 09:00 [pubmed]
PHST- 2008/04/01 09:00 [medline]
PHST- 2008/02/23 09:00 [entrez]
AID - 790775517 [pii]
AID - 10.1080/03008200701818561 [doi]
PST - ppublish
SO  - Connect Tissue Res. 2008;49(1):7-14. doi: 10.1080/03008200701818561.