PMID- 18293954
OWN - NLM
STAT- MEDLINE
DCOM- 20080701
LR  - 20171116
IS  - 1520-6106 (Print)
IS  - 1520-5207 (Linking)
VI  - 112
IP  - 11
DP  - 2008 Mar 20
TI  - Energy transfer photophysics from serum albumins to sequestered 
      3-hydroxy-2-naphthoic acid, an excited state intramolecular proton-transfer 
      probe.
PG  - 3451-61
LID - 10.1021/jp074598+ [doi]
AB  - The steady-state and time-resolved studies of the sensitized emission of the 
      excited-state proton transfer (ESIPT) probe 3-hydroxy-2-naphthoic acid (3HNA) 
      when bound to bovine serum albumin (BSA) and human serum albumin (HSA) indicate 
      that the nonradiative dipole-dipole Forster type energy transfer from Trp singlet 
      state of proteins to the ESIPT singlet state of 3HNA is greater in the case of 
      HSA. This is supported by the distance and the orientation of the donor-acceptor 
      pair obtained from the protein-ligand docking studies. The docking studies of the 
      complex of BSA-3HNA also indicate that Trp 134 rather than Trp 213 is involved in 
      the energy transfer process. The local environment of Trp 134 in BSA rather than 
      that of Trp 213 is perturbed because of interaction with 3HNA as revealed by the 
      optical resolution of Trp 134 phosphorescence in the complex at 77 K. Docking 
      studies support the larger rotational correlation time, thetac (approximately 50 
      ns), observed for Trp residue/residues in the complexes of HSA and BSA compared 
      with that in the free proteins.
FAU - Sardar, Pinki Saha
AU  - Sardar PS
AD  - Department of Chemistry, Presidency College, Calcutta 700 073, India.
FAU - Samanta, Subhodip
AU  - Samanta S
FAU - Maity, Shyam Sundar
AU  - Maity SS
FAU - Dasgupta, Swagata
AU  - Dasgupta S
FAU - Ghosh, Sanjib
AU  - Ghosh S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080223
PL  - United States
TA  - J Phys Chem B
JT  - The journal of physical chemistry. B
JID - 101157530
RN  - 0 (Naphthols)
RN  - 0 (Protons)
RN  - 0 (Serum Albumin)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 8DUH1N11BX (Tryptophan)
RN  - C7S9D784HX (3-hydroxy-2-naphthoic acid)
SB  - IM
MH  - Binding Sites
MH  - Energy Transfer
MH  - Naphthols/*chemistry
MH  - *Photochemistry
MH  - Physical Phenomena
MH  - *Physics
MH  - *Protons
MH  - Serum Albumin/*chemistry
MH  - Serum Albumin, Bovine/chemistry
MH  - Spectrometry, Fluorescence
MH  - Tryptophan/chemistry
EDAT- 2008/02/26 09:00
MHDA- 2008/07/02 09:00
CRDT- 2008/02/26 09:00
PHST- 2008/02/26 09:00 [pubmed]
PHST- 2008/07/02 09:00 [medline]
PHST- 2008/02/26 09:00 [entrez]
AID - 10.1021/jp074598+ [doi]
PST - ppublish
SO  - J Phys Chem B. 2008 Mar 20;112(11):3451-61. doi: 10.1021/jp074598+. Epub 2008 Feb 
      23.