PMID- 18295336
OWN - NLM
STAT- MEDLINE
DCOM- 20080605
LR  - 20131121
IS  - 0161-813X (Print)
IS  - 0161-813X (Linking)
VI  - 29
IP  - 2
DP  - 2008 Mar
TI  - The effects of mercury on muscarinic cholinergic receptor subtypes (M1 and M2) in 
      captive mink.
PG  - 328-34
LID - 10.1016/j.neuro.2008.01.003 [doi]
AB  - A combination of in vitro (competitive binding assays) and in vivo (tissues from 
      animals exposed to dietary methyl mercury, MeHg) experimental procedures was 
      employed to assess the effects of mercury (MeHg, HgCl(2)) on the two-key 
      muscarinic cholinergic (mACh) receptor subtypes (M1, M2) in two brain regions 
      (occipital cortex, brain stem) of captive mink (Mustela vison). In vitro, HgCl(2) 
      and MeHg were equipotent in inhibiting [(3)H]-pirenzipine binding to the M1 
      receptor in the occipital cortex, but in the brain stem, MeHg was about 65x more 
      potent than HgCl(2). For the M2 receptor, both HgCl(2) and MeHg were more potent 
      at inhibiting [(3)H]-AFDX-384 binding in the occipital cortex than in the brain 
      stem. Within each brain region, HgCl(2) was more potent at inhibiting 
      [(3)H]-AFDX-384 binding than MeHg. In vivo exposure of captive mink to MeHg (0.5, 
      1, and 2ppm MeHg in the diet for 89 days) resulted in greater binding of 
      radioligands to the M1 and M2 receptor in the occipital cortex, but not in the 
      brain stem, when compared to control animals. Based on the in vitro results, we 
      could not conclude which mACh receptor subtype or brain region was most sensitive 
      to Hg, but the in vivo findings suggest that Hg preferentially affects mACh 
      receptor subtype (M1 and M2) levels in the occipital cortex. By studying distinct 
      mACh receptors, these results extend upon previous studies in laboratory rodents 
      and wildlife that showed Hg to affect the global population of mACh receptors.
FAU - Basu, Niladri
AU  - Basu N
AD  - Department of Environmental Health Sciences, University of Michigan, Ann Arbor, 
      MI 48109, USA. niladri@umich.edu
FAU - Scheuhammer, Anton M
AU  - Scheuhammer AM
FAU - Rouvinen-Watt, Kirsti
AU  - Rouvinen-Watt K
FAU - Evans, R Douglas
AU  - Evans RD
FAU - Grochowina, Nicole
AU  - Grochowina N
FAU - Chan, Laurie H M
AU  - Chan LH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080119
PL  - Netherlands
TA  - Neurotoxicology
JT  - Neurotoxicology
JID - 7905589
RN  - 0 (Environmental Pollutants)
RN  - 0 (Methylmercury Compounds)
RN  - 0 (Muscarinic Antagonists)
RN  - 0 (Receptor, Muscarinic M1)
RN  - 0 (Receptor, Muscarinic M2)
RN  - 10028-17-8 (Tritium)
RN  - 118290-27-0 (AFDX 384)
RN  - 3G0285N20N (Pirenzepine)
RN  - RWZ4L3O1X0 (methylmercuric chloride)
SB  - IM
MH  - Animals
MH  - Binding, Competitive
MH  - Brain Stem/*drug effects/metabolism
MH  - Diet
MH  - Dose-Response Relationship, Drug
MH  - Environmental Pollutants/*toxicity
MH  - Food Contamination
MH  - Male
MH  - Methylmercury Compounds/*toxicity
MH  - Mink
MH  - Muscarinic Antagonists/metabolism
MH  - Occipital Lobe/*drug effects/metabolism
MH  - Pirenzepine/analogs & derivatives/metabolism
MH  - Radioligand Assay
MH  - Receptor, Muscarinic M1/*drug effects/metabolism
MH  - Receptor, Muscarinic M2/*drug effects/metabolism
MH  - Tritium
EDAT- 2008/02/26 09:00
MHDA- 2008/06/06 09:00
CRDT- 2008/02/26 09:00
PHST- 2007/10/31 00:00 [received]
PHST- 2007/12/06 00:00 [revised]
PHST- 2008/01/10 00:00 [accepted]
PHST- 2008/02/26 09:00 [pubmed]
PHST- 2008/06/06 09:00 [medline]
PHST- 2008/02/26 09:00 [entrez]
AID - S0161-813X(08)00006-5 [pii]
AID - 10.1016/j.neuro.2008.01.003 [doi]
PST - ppublish
SO  - Neurotoxicology. 2008 Mar;29(2):328-34. doi: 10.1016/j.neuro.2008.01.003. Epub 
      2008 Jan 19.