PMID- 18295774
OWN - NLM
STAT- MEDLINE
DCOM- 20080530
LR  - 20131121
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1185
IP  - 2
DP  - 2008 Mar 28
TI  - Trace determination of beta-lactam antibiotics in environmental aqueous samples 
      using off-line and on-line preconcentration in capillary electrophoresis.
PG  - 273-80
LID - 10.1016/j.chroma.2007.12.088 [doi]
AB  - A sensitive and reliable method using capillary zone electrophoresis with 
      UV-diode array detection (CZE-DAD) has been developed and validated for trace 
      determination of beta-lactam antibiotics in waste, well and river water matrices. 
      Due to the lack of sensitivity of the UV-vis detection, a solvent 
      extraction/solid-phase extraction (SPE) method applied for off-line 
      preconcentration and cleanup of water samples, in combination with an on-line 
      preconcentration methodology named large volume sample stacking (LVSS) have been 
      applied. The analytes included nafcillin, dicloxacillin, cloxacillin, oxacillin, 
      ampicillin, penicillin G and amoxicillin. Average recoveries for water samples 
      fortified with the studied beta-lactams at different concentration levels (1.0, 
      2.0 and 4.0 microg/L) were ranging between 94 and 99%, with relative standard 
      deviations (RSDs) lower than 10%. The precision, calculated as intra-day and 
      inter-day standard deviations fell within acceptable ranges (3.3-7.2%). The 
      limits of detection were estimated to range between 0.08 and 0.80 microgL(-1) for 
      the studied compounds. All the samples analyzed were negative for all the 
      analytes at these levels of concentration and the method showed its usefulness 
      for the detection of these widely applied beta-lactam antibiotics in different 
      kinds of waters.
FAU - Bailon-Perez, M I
AU  - Bailon-Perez MI
AD  - Department of Analytical Chemistry, Faculty of Sciences, Campus Fuentenueva, s/n, 
      E-18071 Granada, Spain.
FAU - Garcia-Campana, A M
AU  - Garcia-Campana AM
FAU - Cruces-Blanco, C
AU  - Cruces-Blanco C
FAU - del Olmo Iruela, M
AU  - del Olmo Iruela M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080112
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Water Pollutants, Chemical)
RN  - 0 (beta-Lactams)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Anti-Bacterial Agents/*analysis/chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Environment
MH  - Environmental Monitoring/*methods
MH  - Sensitivity and Specificity
MH  - Solid Phase Extraction/*methods
MH  - Water/*analysis
MH  - Water Pollutants, Chemical/*analysis
MH  - beta-Lactams/*analysis/chemistry
EDAT- 2008/02/26 09:00
MHDA- 2008/05/31 09:00
CRDT- 2008/02/26 09:00
PHST- 2007/09/13 00:00 [received]
PHST- 2007/12/27 00:00 [revised]
PHST- 2007/12/31 00:00 [accepted]
PHST- 2008/02/26 09:00 [pubmed]
PHST- 2008/05/31 09:00 [medline]
PHST- 2008/02/26 09:00 [entrez]
AID - S0021-9673(08)00015-0 [pii]
AID - 10.1016/j.chroma.2007.12.088 [doi]
PST - ppublish
SO  - J Chromatogr A. 2008 Mar 28;1185(2):273-80. doi: 10.1016/j.chroma.2007.12.088. 
      Epub 2008 Jan 12.