PMID- 18297440
OWN - NLM
STAT- MEDLINE
DCOM- 20080506
LR  - 20220330
IS  - 0944-1174 (Print)
IS  - 0944-1174 (Linking)
VI  - 43
IP  - 1
DP  - 2008
TI  - Blocking of monocyte chemoattractant protein-1 (MCP-1) activity attenuates the 
      severity of acute pancreatitis in rats.
PG  - 79-85
LID - 10.1007/s00535-007-2126-9 [doi]
AB  - BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) has been shown to affect 
      the progression of various inflammatory disorders, including pancreatitis. To 
      investigate the role of MCP-1 in acute pancreatitis and to seek possible 
      therapeutic means, we evaluated the effect of a plasmid expression vector 
      containing a dominant-negative mutant MCP-1 gene (mMCP-1). METHODS: Two rat 
      models of acute pancreatitis were employed that used either cerulein (for mild 
      pancreatitis) or a mixture of 5% taurocholic acid and trypsin (for severe 
      pancreatitis). At 6 h after induction of acute pancreatitis with or without 
      injection of mMCP-1, serum amylase levels and cytokine levels, as well as 
      morphological evaluation of the pancreas, were determined. Survival rates were 
      also evaluated. RESULTS: Severe pancreatitis was significantly reduced by mMCP-1 
      injection. mMCP-1 decreased serum levels of amylase, IL-6, IL-10, and LDH, and 
      improved the survival rate 48 h after disease onset. Histopathological changes of 
      pancreas and lungs were also improved by mMCP-1. CONCLUSIONS: MCP-1 appears to be 
      involved in the progression of severe forms of acute pancreatitis. Our data 
      suggested that MCP-1 is a candidate as a therapeutic target to treat acute 
      pancreatitis.
FAU - Ishibashi, Toshiaki
AU  - Ishibashi T
AD  - Department of Medicine and Bioregulatory Science, Graduate School of Medical 
      Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, 
      Japan.
FAU - Zhao, Haifeng
AU  - Zhao H
FAU - Kawabe, Ken
AU  - Kawabe K
FAU - Oono, Takamasa
AU  - Oono T
FAU - Egashira, Kensuke
AU  - Egashira K
FAU - Suzuki, Koichi
AU  - Suzuki K
FAU - Nawata, Hajime
AU  - Nawata H
FAU - Takayanagi, Ryoichi
AU  - Takayanagi R
FAU - Ito, Tetsuhide
AU  - Ito T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080224
PL  - Japan
TA  - J Gastroenterol
JT  - Journal of gastroenterology
JID - 9430794
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 888Y08971B (Ceruletide)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Ceruletide/toxicity
MH  - Chemokine CCL2/*antagonists & inhibitors/blood
MH  - Cytokines
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Genetic Vectors/*administration & dosage
MH  - Injections
MH  - Male
MH  - Pancreas/drug effects/pathology
MH  - Pancreatitis, Acute Necrotizing/blood/chemically induced/*drug therapy
MH  - *Plasmids
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Severity of Illness Index
MH  - Treatment Outcome
EDAT- 2008/02/26 09:00
MHDA- 2008/05/07 09:00
CRDT- 2008/02/26 09:00
PHST- 2007/08/20 00:00 [received]
PHST- 2007/10/08 00:00 [accepted]
PHST- 2008/02/26 09:00 [pubmed]
PHST- 2008/05/07 09:00 [medline]
PHST- 2008/02/26 09:00 [entrez]
AID - 10.1007/s00535-007-2126-9 [doi]
PST - ppublish
SO  - J Gastroenterol. 2008;43(1):79-85. doi: 10.1007/s00535-007-2126-9. Epub 2008 Feb 
      24.