PMID- 18298336
OWN - NLM
STAT- MEDLINE
DCOM- 20080522
LR  - 20191210
IS  - 1084-9785 (Print)
IS  - 1084-9785 (Linking)
VI  - 23
IP  - 1
DP  - 2008 Feb
TI  - Dendritic cells expressing a combined PADRE/MUC4-derived polyepitope DNA vaccine 
      induce multiple cytotoxic T-cell responses.
PG  - 121-8
LID - 10.1089/cbr.2007.0427 [doi]
AB  - The tumor-associated antigen, mucin4 (MUC4), is overexpressed on various 
      epithelial malignancies, making it a potentially broadly applicable candidate 
      vaccine therapy. In this paper, we report on the creation of a dendritic cell 
      (DC)-based vaccine, using cells transduced with the universal DR-restricted Th 
      helper epitope (PADRE) combined with human leukocyte antigen (HLA)-A1- and 
      HLA-A2-restricted epitopes from MUC4 (rAd-pE-DCs). We examined this vaccine's 
      biologic characteristics and immune activity in vitro, finding that infection 
      with the polyepitope adenovirus did not alter the typical morphology of mature DC 
      and the typical markers of these cells (CD86, CD83, CD80, and HLA-DR) were highly 
      expressed on rAd-pE-DCs. Lymphocytes primed with rAd-pE-DCs generated potent 
      cytotoxic responses. By contrast, lymphocytes primed with a GFP-expressing 
      adenovirus (rAd-GFP-DCs) or mock-transfected DCs were not cytotoxic. Transduction 
      of DCs with an adenovirus encoding PADRE combined with HLA-A1- and 
      HLA-A2-restricted epitopes may be a potential strategy for the immunotherapy of 
      MUC4-associated tumors.
FAU - Wei, Jishu
AU  - Wei J
AD  - Department of General Surgery, the First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, People's Republic of China.
FAU - Gao, Wentao
AU  - Gao W
FAU - Wu, Junli
AU  - Wu J
FAU - Meng, Kai
AU  - Meng K
FAU - Zhang, Jingjing
AU  - Zhang J
FAU - Chen, Jianmin
AU  - Chen J
FAU - Miao, Yi
AU  - Miao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Biother Radiopharm
JT  - Cancer biotherapy & radiopharmaceuticals
JID - 9605408
RN  - 0 (Epitopes)
RN  - 0 (MUC4 protein, human)
RN  - 0 (Malaria Vaccines)
RN  - 0 (Mucin-4)
RN  - 0 (Mucins)
RN  - 0 (PADRE 45)
RN  - 0 (Vaccines, DNA)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Base Sequence
MH  - COS Cells
MH  - Chlorocebus aethiops
MH  - Cytotoxicity, Immunologic/*immunology
MH  - Dendritic Cells/*immunology
MH  - Epitopes/immunology
MH  - Genetic Engineering
MH  - Humans
MH  - Interferon-gamma
MH  - Malaria Vaccines/*immunology
MH  - Molecular Sequence Data
MH  - Mucin-4
MH  - Mucins/*immunology
MH  - Neoplasms/*immunology/metabolism/pathology
MH  - Phenotype
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Transduction, Genetic
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Vaccines, DNA/administration & dosage/*immunology
EDAT- 2008/02/27 09:00
MHDA- 2008/05/23 09:00
CRDT- 2008/02/27 09:00
PHST- 2008/02/27 09:00 [pubmed]
PHST- 2008/05/23 09:00 [medline]
PHST- 2008/02/27 09:00 [entrez]
AID - 10.1089/cbr.2007.0427 [doi]
PST - ppublish
SO  - Cancer Biother Radiopharm. 2008 Feb;23(1):121-8. doi: 10.1089/cbr.2007.0427.