PMID- 18298910
OWN - NLM
STAT- MEDLINE
DCOM- 20080403
LR  - 20111117
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 121
IP  - 3
DP  - 2008 Feb 5
TI  - Expression of surface markers on peripheral CD4+CD25high T cells in patients with 
      atopic asthma: role of inhaled corticosteroid.
PG  - 205-12
AB  - BACKGROUND: CD4(+)CD25(+) regulatory T cells (Tregs) mediate immune suppression 
      through cell-cell contact with surface molecules, particularly cytotoxic T 
      lymphocyte-associated antigen 4 (CTLA-4), glucocorticoid-induced tumor necrosis 
      factor receptor family-related protein (GITR), and transforming growth factor 
      beta (TGF-beta), but little is known about the exact role of Tregs in the 
      pathogenesis of asthma. This study sought to characterize the expression of 
      surface markers on peripheral blood mononuclear cells-derived Tregs in patients 
      with atopic asthma and healthy subjects, and to investigate the effect of inhaled 
      corticosteroid on them. METHODS: The expression of surface molecules on 
      CD4(+)CD25(high) Tregs was detected by flow cytometry. The effect of inhaled 
      corticosteroid on expression of the surface molecules on Tregs was determined in 
      vivo and in vitro. Total serum immunoglobulin E (IgE) and high-sensitivity 
      C-reactive protein were measured by enzyme linked immunosorbent assay and latex 
      enhanced immunoturbidimetric assay, respectively. RESULTS: Equivalent numbers of 
      peripheral Tregs were found in patients with atopic asthma (stable and acute) and 
      healthy subjects. Tregs preferentially expressed CTLA-4, GITR, toll-like receptor 
      4 (TLR4), latency-associated peptide (LAP/TGF-beta1), and forkhead box P3 
      (FOXP3). Patients with acute asthma had decreased numbers of 
      CD4(+)CD25(high)LAP(+) T cells compared to healthy subjects and stable 
      asthmatics. Inhaled corticosteroid enhanced the percentage of Tregs expressing 
      LAP in vivo and in vitro dose-dependently. Furthermore, the percentages of Tregs 
      expressing LAP were negatively correlated with total serum IgE levels and 
      severity of asthma, but positively correlated with forced expiratory volume in 
      one second percentage of the predicted value in patients with asthma. 
      CONCLUSIONS: The results suggest that membrane-bound TGF-beta1 is a potential 
      candidate for predicting the severity of asthma, and may contribute to the 
      sustained remission of asthma. Strategies targeting Tregs on their surface 
      markers, especially TGF-beta1, are promising for future therapy of asthma.
FAU - Zhang, Qian
AU  - Zhang Q
AD  - Department of Respiratory Medicine, First Affiliated Hospital, Nanjing Medical 
      University, Nanjing, Jiangsu 210029, China.
FAU - Qian, Fen-hong
AU  - Qian FH
FAU - Liu, Hua
AU  - Liu H
FAU - Zhou, Lin-fu
AU  - Zhou LF
FAU - Huang, Mao
AU  - Huang M
FAU - Zhang, Xi-long
AU  - Zhang XL
FAU - Yin, Kai-sheng
AU  - Yin KS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Adrenal Cortex Hormones)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (CTLA-4 Antigen)
RN  - 0 (CTLA4 protein, human)
RN  - 0 (FOXP3 protein, human)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Glucocorticoid-Induced TNFR-Related Protein)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (TLR4 protein, human)
RN  - 0 (TNFRSF18 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 51333-22-3 (Budesonide)
SB  - IM
MH  - Administration, Inhalation
MH  - Adrenal Cortex Hormones/*administration & dosage
MH  - Adult
MH  - Antigens, CD/blood
MH  - Antigens, Differentiation/blood
MH  - Asthma/drug therapy/*immunology
MH  - Budesonide/pharmacology
MH  - CTLA-4 Antigen
MH  - Female
MH  - Forkhead Transcription Factors/blood
MH  - Glucocorticoid-Induced TNFR-Related Protein
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Receptors, Nerve Growth Factor/blood
MH  - Receptors, Tumor Necrosis Factor/blood
MH  - T-Lymphocytes, Regulatory/drug effects/*immunology
MH  - Toll-Like Receptor 4/blood
MH  - Transforming Growth Factor beta1/blood
EDAT- 2008/02/27 09:00
MHDA- 2008/04/04 09:00
CRDT- 2008/02/27 09:00
PHST- 2008/02/27 09:00 [pubmed]
PHST- 2008/04/04 09:00 [medline]
PHST- 2008/02/27 09:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2008 Feb 5;121(3):205-12.